TY - JOUR
T1 - The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device
AU - Baker, Alexander N.
AU - Richards, Sarah-Jane
AU - Guy, Collette S.
AU - Congdon, Thomas R.
AU - Hasan, Muhammad
AU - Zwetsloot, Alexander J.
AU - Gallo, Angelo
AU - Lewandowski, Józef R.
AU - Stansfeld, Phillip J.
AU - Straube, Anne
AU - Walker, Marc
AU - Chessa, Simona
AU - Pergolizzi, Giulia
AU - Dedola, Simone
AU - Field, Robert A.
AU - Gibson, Matthew I.
N1 - Funding Information:
MIG is supported by the ERC (638661) and Royal Society (Industry Fellowship 191037). The BBSRC MIBTP program (BB/M01116X/1) and Iceni Diagnostics Ltd are thanked for support to A.N.B. UoW, EPSRC (EP/R511808/1), and BBSRC (BB/S506783/1) impact acceleration accounts are thanked. A.J.Z. is funded by the MRC DTP (MR/N014294/1). A.S. is a Wellcome Trust Investigator (200870/Z/16/Z). The Leverhulme Trust are thanked for support (RPG-2019-087). P.J.S. thanks Wellcome (208361/Z/17/Z), the MRC (MR/S009213/1), and BBSRC (BB/P01948X/1, BB/R002517/1, and BB/S003339/1). The Warwick Polymer and Electron Microscopy Research Technology Platforms (Y. Han) are acknowledged for the SEC/EM analysis. Dr C. Biggs is thanked for lateral flow device preparation. We sincerely thank the technical and administrative staff of the UoW who enabled our laboratory to remain open during the COVID-19 pandemic. Prof. Peter Scott is thanked for critically reading this manuscript.
Publisher Copyright:
© 2020 American Chemical Society. All rights reserved.
PY - 2020/11/25
Y1 - 2020/11/25
N2 - There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.
AB - There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.
UR - http://www.scopus.com/inward/record.url?scp=85092375755&partnerID=8YFLogxK
U2 - 10.1021/acscentsci.0c00855
DO - 10.1021/acscentsci.0c00855
M3 - Article
C2 - 33269329
SN - 2374-7943
VL - 6
SP - 2046
EP - 2052
JO - ACS Central Science
JF - ACS Central Science
IS - 11
ER -
