The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device: ACS Central Science

Alexander N. Baker; Sarah-Jane Richards; Collette S. Guy; Thomas R. Congdon; Muhammad Hasan; Alexander J. Zwetsloot; Angelo Gallo; Józef R. Lewandowski; Phillip J. Stansfeld; Anne Straube; Marc Walker; Simona Chessa; Giulia Pergolizzi; Simone Dedola; Robert A. Field; Matthew I. Gibson

doi:10.1021/acscentsci.0c00855

The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device: ACS Central Science

Alexander N. Baker, Sarah-Jane Richards, Collette S. Guy, Thomas R. Congdon, Muhammad Hasan, Alexander J. Zwetsloot, Angelo Gallo, Józef R. Lewandowski, Phillip J. Stansfeld, Anne Straube, Marc Walker, Simona Chessa, Giulia Pergolizzi, Simone Dedola, Robert A. Field, Matthew I. Gibson

Research output: Contribution to journal › Article › peer-review

Abstract

There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.

Original language	English
Journal	ACS Central Science
DOIs	https://doi.org/10.1021/acscentsci.0c00855
Publication status	Published - 22 Sept 2020

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Manchester Institute of Biotechnology

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Baker, A. N., Richards, S-J., Guy, C. S., Congdon, T. R., Hasan, M., Zwetsloot, A. J., Gallo, A., Lewandowski, J. R., Stansfeld, P. J., Straube, A., Walker, M., Chessa, S., Pergolizzi, G., Dedola, S., Field, R. A., & Gibson, M. I. (2020). The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device: ACS Central Science. ACS Central Science. https://doi.org/10.1021/acscentsci.0c00855

@article{9270a0d317e14fe08ca86faa66ae020e,

title = "The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device: ACS Central Science",

abstract = "There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.",

author = "Baker, {Alexander N.} and Sarah-Jane Richards and Guy, {Collette S.} and Congdon, {Thomas R.} and Muhammad Hasan and Zwetsloot, {Alexander J.} and Angelo Gallo and Lewandowski, {J{\'o}zef R.} and Stansfeld, {Phillip J.} and Anne Straube and Marc Walker and Simona Chessa and Giulia Pergolizzi and Simone Dedola and Field, {Robert A.} and Gibson, {Matthew I.}",

year = "2020",

month = sep,

day = "22",

doi = "10.1021/acscentsci.0c00855",

language = "English",

journal = "ACS Central Science",

issn = "2374-7943",

publisher = "American Chemical Society",

}

Baker, AN, Richards, S-J, Guy, CS, Congdon, TR, Hasan, M, Zwetsloot, AJ, Gallo, A, Lewandowski, JR, Stansfeld, PJ, Straube, A, Walker, M, Chessa, S, Pergolizzi, G, Dedola, S, Field, RA & Gibson, MI 2020, 'The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device: ACS Central Science', ACS Central Science. https://doi.org/10.1021/acscentsci.0c00855

TY - JOUR

T1 - The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device

T2 - ACS Central Science

AU - Baker, Alexander N.

AU - Richards, Sarah-Jane

AU - Guy, Collette S.

AU - Congdon, Thomas R.

AU - Hasan, Muhammad

AU - Zwetsloot, Alexander J.

AU - Gallo, Angelo

AU - Lewandowski, Józef R.

AU - Stansfeld, Phillip J.

AU - Straube, Anne

AU - Walker, Marc

AU - Chessa, Simona

AU - Pergolizzi, Giulia

AU - Dedola, Simone

AU - Field, Robert A.

AU - Gibson, Matthew I.

PY - 2020/9/22

Y1 - 2020/9/22

N2 - There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.

AB - There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.

U2 - 10.1021/acscentsci.0c00855

DO - 10.1021/acscentsci.0c00855

M3 - Article

SN - 2374-7943

JO - ACS Central Science

JF - ACS Central Science

ER -

The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device: ACS Central Science

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