The TES gene at 7q31.1 is methylated in tumours and encodes a novel growth-suppressing LIM domain protein

E. S. Tobias, A. F L Hurlstone, E. MacKenzie, R. Mcfarlane, D. M. Black

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Many studies suggest that a multi-tissue tumour suppressor gene is located at human chromosome 7q31.1. We have cloned and characterized a novel gene at this locus. The TES gene lies within the minimal region of overlap of several LOH studies and appears to possess the properties of a tumour suppressor. TES is widely expressed and is predicted to encode a protein of 421 amino acids, with three C-terminal LIM domains. Mutation analysis of the coding TES exons in 21 human tumour-derived cell lines revealed the presence of a frameshift mutation in one allele in the breast cancer cell line ZR-75. Methylation of the CpG island at the 5′ end of TES appears to be a remarkably frequent finding, occurring in seven out of 10 ovarian carcinomas and in each of the 30 tumour-derived cell lines tested. Moreover, forced expression of TES in HeLa or OVCAR5 cells, resulted in a profound reduction in growth potential, as determined by the colony formation assay. We believe that TES is a tumour suppressor gene that is inactivated primarily by transcriptional silencing resulting from CpG island methylation.
    Original languageEnglish
    Pages (from-to)2844-2853
    Number of pages9
    JournalOncogene
    Volume20
    Issue number22
    DOIs
    Publication statusPublished - 17 May 2001

    Keywords

    • Chromosome 7
    • LIM domain
    • Methylation
    • TSG

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