The Trypanosoma cruzi genome contains ion motive ATPase genes which closely resemble Leishmania proton pumps

John C. Meade; Chunling Li; Jonathan K. Stiles; Michelle E. Moate; Jeffrey I. Penny; Sanjeev Krishna; Richard W. Finley

doi:10.1016/S1383-5769(00)00061-1

The Trypanosoma cruzi genome contains ion motive ATPase genes which closely resemble Leishmania proton pumps

John C. Meade, Chunling Li, Jonathan K. Stiles, Michelle E. Moate, Jeffrey I. Penny, Sanjeev Krishna, Richard W. Finley

Research output: Contribution to journal › Article › peer-review

Abstract

DNA fragments homologous to members of the family of P-type ion-motive ATPases were identified in Trypanosoma cruzi by polymerase chain reaction (PCR) amplification. The sequence of one fragment, which closely resembled (87% identity) the tandemly linked proton pumps in Leishmania, was used to characterize the H+-ATPase genes in T. cruzi. The T. cruzi proton pump locus contains four tandemly repeated genes (TCH1-4) separated by 1.1 kb intergenic regions. The nucleotide sequence of one cloned gene of the tandem array contains a 2775 nt open reading frame encoding a predicted 101908-Da protein of 925 amino acids. The TCH genes are expressed as 3.8 and 4.9 kb polyadenylated transcripts in the epimastigote stage; expression of both transcripts is reduced in metacyclic trypomastigotes. Results of 5' and 3' RACE transcript mapping indicate that the 3.8 kb message is generated from within the tandemly repeated locus. The 3.8 kb TCH transcript has the T. cruzi mini-exon appended to a short (40 nt) 5' untranslated region (UTR) and has a 927 nt 3' UTR. The full peptide sequence of the T. cruzi proton pump is 80% identical to the Leishmania pump but lacks the extended carboxyl tail present in the Leishmania ATPase. An antibody that recognizes the 110-kDa Leishmania donovani proton pump cross-reacts with a 100-kDa protein in lysates of T. cruzi epimastigotes. (C) 2000 Elsevier Science Ltd.

Original language	English
Pages (from-to)	309-320
Number of pages	11
Journal	Parasitology International
Volume	49
Issue number	4
DOIs	https://doi.org/10.1016/S1383-5769(00)00061-1
Publication status	Published - 2000

Keywords

Leishmania
P-type ATPase
Proton motive ATPase
Trypanosoma cruzi
Trypanosomatidae

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10.1016/S1383-5769(00)00061-1

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@article{0760e80ee3f242bfb168fa86df3a1ffd,

title = "The Trypanosoma cruzi genome contains ion motive ATPase genes which closely resemble Leishmania proton pumps",

abstract = "DNA fragments homologous to members of the family of P-type ion-motive ATPases were identified in Trypanosoma cruzi by polymerase chain reaction (PCR) amplification. The sequence of one fragment, which closely resembled (87% identity) the tandemly linked proton pumps in Leishmania, was used to characterize the H+-ATPase genes in T. cruzi. The T. cruzi proton pump locus contains four tandemly repeated genes (TCH1-4) separated by 1.1 kb intergenic regions. The nucleotide sequence of one cloned gene of the tandem array contains a 2775 nt open reading frame encoding a predicted 101908-Da protein of 925 amino acids. The TCH genes are expressed as 3.8 and 4.9 kb polyadenylated transcripts in the epimastigote stage; expression of both transcripts is reduced in metacyclic trypomastigotes. Results of 5' and 3' RACE transcript mapping indicate that the 3.8 kb message is generated from within the tandemly repeated locus. The 3.8 kb TCH transcript has the T. cruzi mini-exon appended to a short (40 nt) 5' untranslated region (UTR) and has a 927 nt 3' UTR. The full peptide sequence of the T. cruzi proton pump is 80% identical to the Leishmania pump but lacks the extended carboxyl tail present in the Leishmania ATPase. An antibody that recognizes the 110-kDa Leishmania donovani proton pump cross-reacts with a 100-kDa protein in lysates of T. cruzi epimastigotes. (C) 2000 Elsevier Science Ltd.",

keywords = "Leishmania, P-type ATPase, Proton motive ATPase, Trypanosoma cruzi, Trypanosomatidae",

author = "Meade, {John C.} and Chunling Li and Stiles, {Jonathan K.} and Moate, {Michelle E.} and Penny, {Jeffrey I.} and Sanjeev Krishna and Finley, {Richard W.}",

year = "2000",

doi = "10.1016/S1383-5769(00)00061-1",

language = "English",

volume = "49",

pages = "309--320",

journal = "Parasitology International",

issn = "1383-5769",

publisher = "Elsevier BV",

number = "4",

}

TY - JOUR

T1 - The Trypanosoma cruzi genome contains ion motive ATPase genes which closely resemble Leishmania proton pumps

AU - Meade, John C.

AU - Li, Chunling

AU - Stiles, Jonathan K.

AU - Moate, Michelle E.

AU - Penny, Jeffrey I.

AU - Krishna, Sanjeev

AU - Finley, Richard W.

PY - 2000

Y1 - 2000

N2 - DNA fragments homologous to members of the family of P-type ion-motive ATPases were identified in Trypanosoma cruzi by polymerase chain reaction (PCR) amplification. The sequence of one fragment, which closely resembled (87% identity) the tandemly linked proton pumps in Leishmania, was used to characterize the H+-ATPase genes in T. cruzi. The T. cruzi proton pump locus contains four tandemly repeated genes (TCH1-4) separated by 1.1 kb intergenic regions. The nucleotide sequence of one cloned gene of the tandem array contains a 2775 nt open reading frame encoding a predicted 101908-Da protein of 925 amino acids. The TCH genes are expressed as 3.8 and 4.9 kb polyadenylated transcripts in the epimastigote stage; expression of both transcripts is reduced in metacyclic trypomastigotes. Results of 5' and 3' RACE transcript mapping indicate that the 3.8 kb message is generated from within the tandemly repeated locus. The 3.8 kb TCH transcript has the T. cruzi mini-exon appended to a short (40 nt) 5' untranslated region (UTR) and has a 927 nt 3' UTR. The full peptide sequence of the T. cruzi proton pump is 80% identical to the Leishmania pump but lacks the extended carboxyl tail present in the Leishmania ATPase. An antibody that recognizes the 110-kDa Leishmania donovani proton pump cross-reacts with a 100-kDa protein in lysates of T. cruzi epimastigotes. (C) 2000 Elsevier Science Ltd.

AB - DNA fragments homologous to members of the family of P-type ion-motive ATPases were identified in Trypanosoma cruzi by polymerase chain reaction (PCR) amplification. The sequence of one fragment, which closely resembled (87% identity) the tandemly linked proton pumps in Leishmania, was used to characterize the H+-ATPase genes in T. cruzi. The T. cruzi proton pump locus contains four tandemly repeated genes (TCH1-4) separated by 1.1 kb intergenic regions. The nucleotide sequence of one cloned gene of the tandem array contains a 2775 nt open reading frame encoding a predicted 101908-Da protein of 925 amino acids. The TCH genes are expressed as 3.8 and 4.9 kb polyadenylated transcripts in the epimastigote stage; expression of both transcripts is reduced in metacyclic trypomastigotes. Results of 5' and 3' RACE transcript mapping indicate that the 3.8 kb message is generated from within the tandemly repeated locus. The 3.8 kb TCH transcript has the T. cruzi mini-exon appended to a short (40 nt) 5' untranslated region (UTR) and has a 927 nt 3' UTR. The full peptide sequence of the T. cruzi proton pump is 80% identical to the Leishmania pump but lacks the extended carboxyl tail present in the Leishmania ATPase. An antibody that recognizes the 110-kDa Leishmania donovani proton pump cross-reacts with a 100-kDa protein in lysates of T. cruzi epimastigotes. (C) 2000 Elsevier Science Ltd.

KW - Leishmania

KW - P-type ATPase

KW - Proton motive ATPase

KW - Trypanosoma cruzi

KW - Trypanosomatidae

U2 - 10.1016/S1383-5769(00)00061-1

DO - 10.1016/S1383-5769(00)00061-1

M3 - Article

SN - 1383-5769

VL - 49

SP - 309

EP - 320

JO - Parasitology International

JF - Parasitology International

IS - 4

ER -

The Trypanosoma cruzi genome contains ion motive ATPase genes which closely resemble Leishmania proton pumps

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Keywords

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