The type II interleukin-1 receptor (IL-1RII) of the bony fish gilthead seabream Sparus aurata is strongly induced after infection and tightly regulated at transcriptional and post-transcriptional levels

Gloria López-Castejón, M. Pilar Sepulcre, Francisco J. Roca, Barbara Castellana, Josep V. Planas, José Meseguer, Victoriano Mulero

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Interleukin-1β (IL-1β) is the prototypic pro-inflammatory cytokine. All the biological effects of IL-1β are mediated through interaction with type 1 IL-1 receptor (IL-1RI), whereas another receptor, called type 2 IL-1R (IL-1RII), lacks an intracellular signalling domain and acts as a decoy receptor that down-regulates responses to IL-1β. Although both receptors are present in bony fish, their expression and biological role in the regulation of IL-1β activity in non-mammalian vertebrates remain to be established. In this study, a homologue of mammalian IL-1RII was isolated and characterized in the gilthead seabream (Sparus aurata). The seabream IL-1RII harboured two Ig-like domains in its extracellular region and a short cytoplasmic tail lacking a signalling domain. The seabream IL-1RII cDNA showed an unexpectedly long 3′UTR compared with that from other species and contained three ATTTA instability motifs, which seem to be responsible for its relatively short half-life (less than 2 h). The expression of seabream IL-1RII was dramatically up-regulated after infection with Vibrio anguillarum in all the immune tissues examined and was even more strongly induced than the IL-1β gene in the head kidney, spleen and liver. Strikingly, the mRNA levels of IL-1RII were 15-fold higher than those of IL-1β in the liver, suggesting a role for this organ in the neutralization of IL-1β leaking into the systemic circulation from the sites of inflammation. In vitro, bacterial DNA and flagellin increased the mRNA levels of IL-1RII in macrophages, while only flagellin was able to weakly induce its expression in acidophilic granulocytes. Finally, the seabream IL-1RII was localized in the plasma membrane when expressed in HEK293 cells and was able to bind IL-1β. © 2006 Elsevier Ltd. All rights reserved.
    Original languageEnglish
    Pages (from-to)2772-2780
    Number of pages8
    JournalMolecular immunology
    Volume44
    Issue number10
    DOIs
    Publication statusPublished - Apr 2007

    Keywords

    • ARE
    • Decoy receptors
    • Fish
    • IL-1
    • mRNA stability
    • Phylogeny

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