TY - JOUR
T1 - The use of ELISA is comparable to immunoprecipitation in the detection of selected myositis-specific autoantibodies in a European population
AU - Loganathan, Aravinthan
AU - McMorrow, Fionnuala
AU - Lu, Hui
AU - Li, Danyang
AU - Mulhearn, Ben
AU - McHugh, Neil John
AU - Tansley, Sarah Louise
N1 - Funding Information:
This work was supported by funding from CureJM and the Bath Institute of Rheumatic Diseases. ELISA kits were provided by MBL. AL’s position at the Royal National Hospital of Rheumatic Diseases is part-funded by the Ken Muirden Fellowship from Arthritis Australia.
Publisher Copyright:
Copyright © 2022 Loganathan, McMorrow, Lu, Li, Mulhearn, McHugh and Tansley.
PY - 2022/9/8
Y1 - 2022/9/8
N2 - BACKGROUND: The reliable detection of myositis-specific autoantibodies (MSA) provides valuable clinical information regarding prognosis, clinical progression and diagnostic confirmation.OBJECTIVES: To evaluate the reliability of a commercial ELISA immunoassay in detecting myositis-specific autoantibodies in comparison to immunoprecipitation as the reference standard.METHODS: Serum samples were chosen from a biobank of more than 3000 samples. Samples with a confirmed MSA on Immunoprecipitation (n=116) were evaluated in duplicate by ELISA to detect Mi2, MDA5, Jo1, EJ, KS, PL-7 and PL-12 (Medical & Biological Laboratories Co. Ltd, Nagoya, Aichi, Japan). Healthy control samples (n=246) confirmed autoantibody negative by immunoprecipitation were similarly assessed.RESULTS: There was a very good agreement between ELISA and immunoprecipitation for serum samples containing anti-Mi2, MDA5, Jo1, EJ, KS and PL-7 and PL-12 auto-antibodies. Cohen's κ values ranged from 0.86-1 for the measured autoantibodies on ELISA.CONCLUSION: ELISA was an accurate method for detecting anti-synthetase, anti-Mi2 and anti-MDA5 autoantibodies.
AB - BACKGROUND: The reliable detection of myositis-specific autoantibodies (MSA) provides valuable clinical information regarding prognosis, clinical progression and diagnostic confirmation.OBJECTIVES: To evaluate the reliability of a commercial ELISA immunoassay in detecting myositis-specific autoantibodies in comparison to immunoprecipitation as the reference standard.METHODS: Serum samples were chosen from a biobank of more than 3000 samples. Samples with a confirmed MSA on Immunoprecipitation (n=116) were evaluated in duplicate by ELISA to detect Mi2, MDA5, Jo1, EJ, KS, PL-7 and PL-12 (Medical & Biological Laboratories Co. Ltd, Nagoya, Aichi, Japan). Healthy control samples (n=246) confirmed autoantibody negative by immunoprecipitation were similarly assessed.RESULTS: There was a very good agreement between ELISA and immunoprecipitation for serum samples containing anti-Mi2, MDA5, Jo1, EJ, KS and PL-7 and PL-12 auto-antibodies. Cohen's κ values ranged from 0.86-1 for the measured autoantibodies on ELISA.CONCLUSION: ELISA was an accurate method for detecting anti-synthetase, anti-Mi2 and anti-MDA5 autoantibodies.
KW - Antibodies, Antinuclear
KW - Autoantibodies
KW - Enzyme-Linked Immunosorbent Assay/methods
KW - Humans
KW - Immunoprecipitation
KW - Myositis
KW - Reproducibility of Results
U2 - 10.3389/fimmu.2022.975939
DO - 10.3389/fimmu.2022.975939
M3 - Article
C2 - 36177007
SN - 1664-3224
VL - 13
JO - Frontiers in Immunology
JF - Frontiers in Immunology
M1 - 975939
ER -