The use of flow cytometry to examine calcium signalling by TRPV1 in mixed cell populations

Bakri M. Assas, Wesam H. Abdulaal, Majed H. Wakid, Haytham A. Zakai, J. Miyan, Joanne Pennock

Research output: Contribution to journalArticlepeer-review

Abstract

Flow cytometric analysis of calcium mobilisation has been in use for many years in the study of specific receptor engagement or isolated cell:cell communication. However, calcium mobilisation/signaling is key to many cell functions including apoptosis, mobility and immune responses. Here we combine multiplex surface staining of whole spleen with Indo-1 AM to visualise calcium mobilisation and examine calcium signaling in a mixed immune cell culture over time. We demonstrate responses to a TRPV1 agonist in distinct cell subtypes without the need for cell separation. Multi parameter staining alongside Indo-1 AM to demonstrate calcium mobilization allows the study of real time calcium signaling in a complex environment
Original languageEnglish
Pages (from-to)13-19
Number of pages7
JournalAnalytical Biochemistry
Volume527
Early online date31 Mar 2017
DOIs
Publication statusPublished - 15 Jun 2017

Keywords

  • Calcium signaling
  • Flow cytometry
  • Immune phenotyping
  • Indo-1 AM
  • TRPV1

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