THOC5 couples M-CSF receptor signaling to transcription factor expression

Louise Carney; Andrew Pierce; Marco Rijnen; M. Belen Gonzalez Sanchez; Hajja G. Hamzah; Liqun Zhang; Teruko Tamura; Anthony D. Whetton

doi:10.1016/j.cellsig.2008.10.018

THOC5 couples M-CSF receptor signaling to transcription factor expression

Louise Carney, Andrew Pierce, Marco Rijnen, M. Belen Gonzalez Sanchez, Hajja G. Hamzah, Liqun Zhang, Teruko Tamura, Anthony D. Whetton

Research output: Contribution to journal › Article › peer-review

Abstract

THOC5 is a nuclear/cytoplasmic protein member of the spliceosome complex which potentiates C/EBP expression in adipocyte differentiation. As C/EBP family members are important regulators of myelopoiesis and THOC5 is highly expressed in neutrophil/macrophage progenitor cells we assessed the role of THOC5 in cytokine-stimulated monocytic development. M-CSF stimulated maturation of the NFS60 cell line was associated with enhanced THOC5 expression and phosphorylation. THOC5 was also shown to form a complex with C/EBPβ. Ectopic expression of THOC5 mimicked M-CSF mediated cell maturation and enhanced protein expression of the myeloid transcription factors C/EBPβ, C/EBPα, Pu-1 and also GAB2 (a PI-3 Kinase and macrophage development regulator). Increased THOC5 expression also mimicked M-CSF stimulated increases in the lipid second messenger PtdInsP3. Inhibition of THOC5-induced increases in PtdInsP3 levels abrogated the elevated levels of C/EBPβ. Thus THOC5 expression can potentiate receptor signalling to transcription factor expression and monocyte differentiation. © 2008 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	309-316
Number of pages	7
Journal	Cell. Signal.
Volume	21
Issue number	2
DOIs	https://doi.org/10.1016/j.cellsig.2008.10.018 https://doi.org/10.1016/j.cellsig.2008.10.018
Publication status	Published - Feb 2009

Keywords

THOC5
C/EBP beta
PI3Kinase
Differentiation
Monocyte
Spliceosome

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{52e6403a3c80454187409af8ca74ba04,

title = "THOC5 couples M-CSF receptor signaling to transcription factor expression",

abstract = "THOC5 is a nuclear/cytoplasmic protein member of the spliceosome complex which potentiates C/EBP expression in adipocyte differentiation. As C/EBP family members are important regulators of myelopoiesis and THOC5 is highly expressed in neutrophil/macrophage progenitor cells we assessed the role of THOC5 in cytokine-stimulated monocytic development. M-CSF stimulated maturation of the NFS60 cell line was associated with enhanced THOC5 expression and phosphorylation. THOC5 was also shown to form a complex with C/EBPβ. Ectopic expression of THOC5 mimicked M-CSF mediated cell maturation and enhanced protein expression of the myeloid transcription factors C/EBPβ, C/EBPα, Pu-1 and also GAB2 (a PI-3 Kinase and macrophage development regulator). Increased THOC5 expression also mimicked M-CSF stimulated increases in the lipid second messenger PtdInsP3. Inhibition of THOC5-induced increases in PtdInsP3 levels abrogated the elevated levels of C/EBPβ. Thus THOC5 expression can potentiate receptor signalling to transcription factor expression and monocyte differentiation. {\textcopyright} 2008 Elsevier Inc. All rights reserved.",

keywords = "THOC5, C/EBP beta, PI3Kinase, Differentiation, Monocyte, Spliceosome",

author = "Louise Carney and Andrew Pierce and Marco Rijnen and Sanchez, {M. Belen Gonzalez} and Hamzah, {Hajja G.} and Liqun Zhang and Teruko Tamura and Whetton, {Anthony D.}",

year = "2009",

month = feb,

doi = "10.1016/j.cellsig.2008.10.018",

language = "English",

volume = "21",

pages = "309--316",

journal = "Cell. Signal.",

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TY - JOUR

T1 - THOC5 couples M-CSF receptor signaling to transcription factor expression

AU - Carney, Louise

AU - Pierce, Andrew

AU - Rijnen, Marco

AU - Sanchez, M. Belen Gonzalez

AU - Hamzah, Hajja G.

AU - Zhang, Liqun

AU - Tamura, Teruko

AU - Whetton, Anthony D.

PY - 2009/2

Y1 - 2009/2

N2 - THOC5 is a nuclear/cytoplasmic protein member of the spliceosome complex which potentiates C/EBP expression in adipocyte differentiation. As C/EBP family members are important regulators of myelopoiesis and THOC5 is highly expressed in neutrophil/macrophage progenitor cells we assessed the role of THOC5 in cytokine-stimulated monocytic development. M-CSF stimulated maturation of the NFS60 cell line was associated with enhanced THOC5 expression and phosphorylation. THOC5 was also shown to form a complex with C/EBPβ. Ectopic expression of THOC5 mimicked M-CSF mediated cell maturation and enhanced protein expression of the myeloid transcription factors C/EBPβ, C/EBPα, Pu-1 and also GAB2 (a PI-3 Kinase and macrophage development regulator). Increased THOC5 expression also mimicked M-CSF stimulated increases in the lipid second messenger PtdInsP3. Inhibition of THOC5-induced increases in PtdInsP3 levels abrogated the elevated levels of C/EBPβ. Thus THOC5 expression can potentiate receptor signalling to transcription factor expression and monocyte differentiation. © 2008 Elsevier Inc. All rights reserved.

AB - THOC5 is a nuclear/cytoplasmic protein member of the spliceosome complex which potentiates C/EBP expression in adipocyte differentiation. As C/EBP family members are important regulators of myelopoiesis and THOC5 is highly expressed in neutrophil/macrophage progenitor cells we assessed the role of THOC5 in cytokine-stimulated monocytic development. M-CSF stimulated maturation of the NFS60 cell line was associated with enhanced THOC5 expression and phosphorylation. THOC5 was also shown to form a complex with C/EBPβ. Ectopic expression of THOC5 mimicked M-CSF mediated cell maturation and enhanced protein expression of the myeloid transcription factors C/EBPβ, C/EBPα, Pu-1 and also GAB2 (a PI-3 Kinase and macrophage development regulator). Increased THOC5 expression also mimicked M-CSF stimulated increases in the lipid second messenger PtdInsP3. Inhibition of THOC5-induced increases in PtdInsP3 levels abrogated the elevated levels of C/EBPβ. Thus THOC5 expression can potentiate receptor signalling to transcription factor expression and monocyte differentiation. © 2008 Elsevier Inc. All rights reserved.

KW - THOC5

KW - C/EBP beta

KW - PI3Kinase

KW - Differentiation

KW - Monocyte

KW - Spliceosome

U2 - 10.1016/j.cellsig.2008.10.018

DO - 10.1016/j.cellsig.2008.10.018

M3 - Article

C2 - 19015024

SN - 0898-6568

VL - 21

SP - 309

EP - 316

JO - Cell. Signal.

JF - Cell. Signal.

IS - 2

ER -

THOC5 couples M-CSF receptor signaling to transcription factor expression

Abstract

Keywords

UN SDGs

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