Three-dimensional image reconstruction of distribution of Pnmt+ cell-derived cells in murine heart

Haibo Ni, Yange Wang, William Crawford, Shanzhuo Zhang, Longxian Cheng, Henggui Zhang, Ming Lei

    Research output: Contribution to journalArticlepeer-review


    Elucidating the function of specific cell types in a highly complex multicellular system such as the heart often requires detailed anatomic reconstruction. We recently described a distinctive class of phenylethanolamine n-methyltransferase (Pnmt+) cell-derived cardiomyocytes (PdCMs), a new cardiomyocyte population with a potential endocrine role. In this dataset, a 3D reconstruction was carried out to visualise the distribution of PdCMs throughout the murine heart. Rigid registration (stiff rotation and translation) was applied to properly align the fused heart slice images based on landmarks using TrakEM2, an open source plug-in in Fiji. The registered slices were then analysed and reconstructed using MATLAB (MATLAB ®. Version The final reconstructed 3D volume was 561×866×48 pixels (corresponding to spatial resolutions of 5.8, 8.9 and 2.5 mm in the x-, y- and z-direction respectively), and visualised in Paraview. The reconstruction allows for detailed analyses of morphology, projections and cellular features of different cell types, enabling further geometrical and topological analyses. Image data can be accessed and viewed through Figshare.

    Original languageEnglish
    Article number170134
    JournalScientific Data
    Publication statusPublished - 26 Sept 2017


    Dive into the research topics of 'Three-dimensional image reconstruction of distribution of Pnmt+ cell-derived cells in murine heart'. Together they form a unique fingerprint.

    Cite this