Three-Dimensional Structure of the Intercalated Disc Reveals Plicate Domain and Gap Junction Remodeling in Heart Failure

Christian Pinali, Hayley Bennett, Bernard Davenport, Jessica Caldwell, Tobias Starborg, Andrew W Trafford, Ashraf Kitmitto

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The intercalated disc (ICD) orchestrates electrochemical and mechanical communication between neighboring cardiac myocytes, properties that are perturbed in heart failure (HF). Although structural data from transmission electron microscopy two-dimensional images have provided valuable insights into the domains forming the ICD, there are currently no three-dimensional (3D) reconstructions for an entire ICD in healthy or diseased hearts. Here, we aimed to understand the link between changes in protein expression in an ovine tachypacing-induced HF model and ultrastructural remodeling of the ICD by determining the 3D intercalated disc architecture using serial block face scanning electron microscopy. In the failing myocardium there is no change to the number of ICDs within the left ventricle, but there is an almost doubling of the number of discs with a surface area of
    Original languageEnglish
    Pages (from-to)498-507
    Number of pages10
    JournalBIOPHYSICAL JOURNAL
    Volume108
    Issue number3
    DOIs
    Publication statusPublished - 3 Feb 2015

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