Three-Dimensional Structure of the Intercalated Disc Reveals Plicate Domain and Gap Junction Remodeling in Heart Failure

Christian Pinali; Hayley Bennett; Bernard Davenport; Jessica Caldwell; Tobias Starborg; Andrew W Trafford; Ashraf Kitmitto

doi:10.1016/j.bpj.2014.12.001

Three-Dimensional Structure of the Intercalated Disc Reveals Plicate Domain and Gap Junction Remodeling in Heart Failure

Christian Pinali, Hayley Bennett, Bernard Davenport, Jessica Caldwell, Tobias Starborg, Andrew W Trafford, Ashraf Kitmitto

Research output: Contribution to journal › Article › peer-review

Abstract

The intercalated disc (ICD) orchestrates electrochemical and mechanical communication between neighboring cardiac myocytes, properties that are perturbed in heart failure (HF). Although structural data from transmission electron microscopy two-dimensional images have provided valuable insights into the domains forming the ICD, there are currently no three-dimensional (3D) reconstructions for an entire ICD in healthy or diseased hearts. Here, we aimed to understand the link between changes in protein expression in an ovine tachypacing-induced HF model and ultrastructural remodeling of the ICD by determining the 3D intercalated disc architecture using serial block face scanning electron microscopy. In the failing myocardium there is no change to the number of ICDs within the left ventricle, but there is an almost doubling of the number of discs with a surface area of

Original language	English
Pages (from-to)	498-507
Number of pages	10
Journal	BIOPHYSICAL JOURNAL
Volume	108
Issue number	3
DOIs	https://doi.org/10.1016/j.bpj.2014.12.001
Publication status	Published - 3 Feb 2015

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title = "Three-Dimensional Structure of the Intercalated Disc Reveals Plicate Domain and Gap Junction Remodeling in Heart Failure",

abstract = "The intercalated disc (ICD) orchestrates electrochemical and mechanical communication between neighboring cardiac myocytes, properties that are perturbed in heart failure (HF). Although structural data from transmission electron microscopy two-dimensional images have provided valuable insights into the domains forming the ICD, there are currently no three-dimensional (3D) reconstructions for an entire ICD in healthy or diseased hearts. Here, we aimed to understand the link between changes in protein expression in an ovine tachypacing-induced HF model and ultrastructural remodeling of the ICD by determining the 3D intercalated disc architecture using serial block face scanning electron microscopy. In the failing myocardium there is no change to the number of ICDs within the left ventricle, but there is an almost doubling of the number of discs with a surface area of",

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T1 - Three-Dimensional Structure of the Intercalated Disc Reveals Plicate Domain and Gap Junction Remodeling in Heart Failure

AU - Pinali, Christian

AU - Bennett, Hayley

AU - Davenport, Bernard

AU - Caldwell, Jessica

AU - Starborg, Tobias

AU - Trafford, Andrew W

AU - Kitmitto, Ashraf

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Three-Dimensional Structure of the Intercalated Disc Reveals Plicate Domain and Gap Junction Remodeling in Heart Failure

Abstract

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