Three novel pigmentation mutants generated by genome-wide random ENU mutagenesis in the mouse

John Curtin, Vicky Tsipouri, John A. Curtin, Pat M. Nolan, Lucie Vizor, Claire A. Parsons, Colin M. Clapham, Ian D. Latham, Lesley J. Rooke, Jo E. Martin, Jo Peters, A. Jakie Hunter, Derek Rogers, Sohaila Rastan, Steve D M Brown, Elizabeth M C Fisher, Nigel K. Spurr N.K., Ian C. Gray

    Research output: Contribution to journalArticlepeer-review


    Three mutant mice with pigmentation phenotypes were recovered from a genome-wide random mouse chemical mutagenesis study. White toes (Whto; MGI:1861986), Belly spot and white toes (Bswt; MGI:2152776) and Dark footpads 2 (Dfp2; MGI:1861991) were identified following visual inspection of progeny from a male exposed to the point mutagen ethylnitrosourea (ENU). In order to rapidly localize the causative mutations, genome-wide linkage scans were performed on pooled DNA samples from backcross animals for each mutant line. Whto was mapped to proximal mouse chromosome (Mmu) 7 between Cen (the centromere) and D7Mit112 (8.0 cM from the centromere), Bswt was mapped to centric Mmu1 between D1Mit214 (32.1 cM) and D1Mit480 (32.8 cM) and Dfp2 was mapped to proximal Mmu4 between Cen and D4Mit18 (5.2 cM). Whto, Bswt and Dfp2 may provide novel starting points in furthering the elucidation of genetic and biochemical pathways relevant to pigmentation and associated biological processes. Copyright © 2004 John Wiley & Sons, Ltd.
    Original languageEnglish
    Pages (from-to)123-127
    Number of pages4
    JournalComparative and Functional Genomics
    Issue number2
    Publication statusPublished - Mar 2004


    • Ethylnitrosourea
    • Linkage
    • Mouse
    • Mutagenesis
    • Pigmentation mutant


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