TY - JOUR
T1 - Three ulcerative colitis susceptibility loci are associated with primary sclerosing cholangitis and indicate a role for IL2, REL, and CARD9
AU - Janse, Marcel
AU - Lamberts, Laetitia E.
AU - Franke, Lude
AU - Raychaudhuri, Soumya
AU - Ellinghaus, Eva
AU - Muri Boberg, Kirsten
AU - Melum, Espen
AU - Folseraas, Trine
AU - Schrumpf, Erik
AU - Bergquist, Annika
AU - Björnsson, Einar
AU - Fu, Jingyuan
AU - Jan Westra, Harm
AU - Groen, Harry J M
AU - Fehrmann, Rudolf S N
AU - Smolonska, Joanna
AU - van den Berg, Leonard H.
AU - Ophoff, Roel A.
AU - Porte, Robert J.
AU - Weismüller, Tobias J.
AU - Wedemeyer, Jochen
AU - Schramm, Christoph
AU - Sterneck, Martina
AU - Günther, Rainer
AU - Braun, Felix
AU - Vermeire, Severine
AU - Henckaerts, Liesbet
AU - Wijmenga, Cisca
AU - Ponsioen, Cyriel Y.
AU - Schreiber, Stefan
AU - Karlsen, Tom H.
AU - Franke, Andre
AU - Weersma, Rinse K.
N1 - 1K08AR055688, NIAMS NIH HHS, United StatesK08 AR055688-04, NIAMS NIH HHS, United StatesR01 NS058980, NINDS NIH HHS, United States
PY - 2011/6
Y1 - 2011/6
N2 - Primary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease characterized by inflammation and fibrosis of the bile ducts. Both environmental and genetic factors contribute to its pathogenesis. To further clarify its genetic background, we investigated susceptibility loci recently identified for ulcerative colitis (UC) in a large cohort of 1,186 PSC patients and 1,748 controls. Single nucleotide polymorphisms (SNPs) tagging 13 UC susceptibility loci were initially genotyped in 854 PSC patients and 1,491 controls from Benelux (331 cases, 735 controls), Germany (265 cases, 368 controls), and Scandinavia (258 cases, 388 controls). Subsequently, a joint analysis was performed with an independent second Scandinavian cohort (332 cases, 257 controls). SNPs at chromosomes 2p16 (P-value 4.12 × 10-4), 4q27 (P-value 4.10 × 10-5), and 9q34 (P-value 8.41 × 10-4) were associated with PSC in the joint analysis after correcting for multiple testing. In PSC patients without inflammatory bowel disease (IBD), SNPs at 4q27 and 9q34 were nominally associated (P <0.05). We applied additional in silico analyses to identify likely candidate genes at PSC susceptibility loci. To identify nonrandom, evidence-based links we used GRAIL (Gene Relationships Across Implicated Loci) analysis showing interconnectivity between genes in six out of in total nine PSC-associated regions. Expression quantitative trait analysis from 1,469 Dutch and UK individuals demonstrated that five out of nine SNPs had an effect on cis-gene expression. These analyses prioritized IL2, CARD9, and REL as novel candidates. Conclusion: We have identified three UC susceptibility loci to be associated with PSC, harboring the putative candidate genes REL, IL2, and CARD9. These results add to the scarce knowledge on the genetic background of PSC and imply an important role for both innate and adaptive immunological factors. Copyright © 2011 American Association for the Study of Liver Diseases.
AB - Primary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease characterized by inflammation and fibrosis of the bile ducts. Both environmental and genetic factors contribute to its pathogenesis. To further clarify its genetic background, we investigated susceptibility loci recently identified for ulcerative colitis (UC) in a large cohort of 1,186 PSC patients and 1,748 controls. Single nucleotide polymorphisms (SNPs) tagging 13 UC susceptibility loci were initially genotyped in 854 PSC patients and 1,491 controls from Benelux (331 cases, 735 controls), Germany (265 cases, 368 controls), and Scandinavia (258 cases, 388 controls). Subsequently, a joint analysis was performed with an independent second Scandinavian cohort (332 cases, 257 controls). SNPs at chromosomes 2p16 (P-value 4.12 × 10-4), 4q27 (P-value 4.10 × 10-5), and 9q34 (P-value 8.41 × 10-4) were associated with PSC in the joint analysis after correcting for multiple testing. In PSC patients without inflammatory bowel disease (IBD), SNPs at 4q27 and 9q34 were nominally associated (P <0.05). We applied additional in silico analyses to identify likely candidate genes at PSC susceptibility loci. To identify nonrandom, evidence-based links we used GRAIL (Gene Relationships Across Implicated Loci) analysis showing interconnectivity between genes in six out of in total nine PSC-associated regions. Expression quantitative trait analysis from 1,469 Dutch and UK individuals demonstrated that five out of nine SNPs had an effect on cis-gene expression. These analyses prioritized IL2, CARD9, and REL as novel candidates. Conclusion: We have identified three UC susceptibility loci to be associated with PSC, harboring the putative candidate genes REL, IL2, and CARD9. These results add to the scarce knowledge on the genetic background of PSC and imply an important role for both innate and adaptive immunological factors. Copyright © 2011 American Association for the Study of Liver Diseases.
U2 - 10.1002/hep.24307
DO - 10.1002/hep.24307
M3 - Article
C2 - 21425313
SN - 0270-9139
VL - 53
SP - 1977
EP - 1985
JO - Hepatology
JF - Hepatology
IS - 6
ER -