TY - JOUR
T1 - Tight binding of a copper (II) phthalocyanine (Cuprolinic Blue) to DNA
AU - John, David C.A.
AU - Rosamund, John
AU - Douglas, Kenneth T.
PY - 1989/3/31
Y1 - 1989/3/31
N2 - The binding of a cationic phthalocyanine (Cuprolinic Blue) to calf thymus DNA, indicated by the increase in the DNA melting temperature and spectroscopic titration (Kaff > 107M-1), was characterised by at least two distinct DNA-bound ligand forms possibly arising from intercalated and externally bound species each with Kaff values in the region 107 M-1. Evidence of strong intercalation was provided by gel electrophoresis of plasmid DNA in the presence of Cuprolinic Blue. The anionic phthalocyanine (copper phthalocyanine 3, 4′, 4″, 4‴-tetrasulfonic acid) does not bind to DNA by spectral criteria, reflecting electrostatic contributions to binding.
AB - The binding of a cationic phthalocyanine (Cuprolinic Blue) to calf thymus DNA, indicated by the increase in the DNA melting temperature and spectroscopic titration (Kaff > 107M-1), was characterised by at least two distinct DNA-bound ligand forms possibly arising from intercalated and externally bound species each with Kaff values in the region 107 M-1. Evidence of strong intercalation was provided by gel electrophoresis of plasmid DNA in the presence of Cuprolinic Blue. The anionic phthalocyanine (copper phthalocyanine 3, 4′, 4″, 4‴-tetrasulfonic acid) does not bind to DNA by spectral criteria, reflecting electrostatic contributions to binding.
UR - https://www.scopus.com/pages/publications/0024521952
U2 - 10.1016/0006-291X(89)92245-6
DO - 10.1016/0006-291X(89)92245-6
M3 - Article
C2 - 2930561
AN - SCOPUS:0024521952
SN - 0006-291X
VL - 159
SP - 1256
EP - 1262
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -