Time-domain fluorescence lifetime imaging applied to biological tissue

Dan Elson; Jose Requejo-Isidro; Ian Munro; Fred Reavell; Jan Siegel; Klaus Suhling; Paul Tadrous; Richard Benninger; Peter Lanigan; James McGinty; Clifford Talbot; Bebhinn Treanor; Stephen Webb; Ann Sandison; Andrew Wallace; Dan Davis; John Lever; Mark Neil; David Philips; Gordon Stamp; Paul French

doi:10.1039/b316456j

Time-domain fluorescence lifetime imaging applied to biological tissue

Dan Elson, Jose Requejo-Isidro, Ian Munro, Fred Reavell, Jan Siegel, Klaus Suhling, Paul Tadrous, Richard Benninger, Peter Lanigan, James McGinty, Clifford Talbot, Bebhinn Treanor, Stephen Webb, Ann Sandison, Andrew Wallace, Dan Davis, John Lever, Mark Neil, David Philips, Gordon StampPaul French

Research output: Contribution to journal › Article › peer-review

Abstract

Fluorescence lifetime imaging (FLIM) is a functional imaging methodology that can provide information, not only concerning the localisation of specific fluorophores, but also about the local fluorophore environment. It may be implemented in scanning confocal or multi-photon microscopes, or in wide-field microscopes and endoscopes. When applied to tissue autofluorescence, it reveals intrinsic excellent contrast between different types and states of tissue. This article aims to review our recent progress in developing time-domain FLIM technology for microscopy and endoscopy and applying it to biological tissue. © The Royal Society of Chemistry and Owner Societies 2004.

Original language	English
Pages (from-to)	795-801
Number of pages	6
Journal	Photochemical and Photobiological Sciences
Volume	3
Issue number	8
DOIs	https://doi.org/10.1039/b316456j
Publication status	Published - Aug 2004

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Elson, D., Requejo-Isidro, J., Munro, I., Reavell, F., Siegel, J., Suhling, K., Tadrous, P., Benninger, R., Lanigan, P., McGinty, J., Talbot, C., Treanor, B., Webb, S., Sandison, A., Wallace, A., Davis, D., Lever, J., Neil, M., Philips, D., ... French, P. (2004). Time-domain fluorescence lifetime imaging applied to biological tissue. Photochemical and Photobiological Sciences, 3(8), 795-801. https://doi.org/10.1039/b316456j

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title = "Time-domain fluorescence lifetime imaging applied to biological tissue",

abstract = "Fluorescence lifetime imaging (FLIM) is a functional imaging methodology that can provide information, not only concerning the localisation of specific fluorophores, but also about the local fluorophore environment. It may be implemented in scanning confocal or multi-photon microscopes, or in wide-field microscopes and endoscopes. When applied to tissue autofluorescence, it reveals intrinsic excellent contrast between different types and states of tissue. This article aims to review our recent progress in developing time-domain FLIM technology for microscopy and endoscopy and applying it to biological tissue. {\textcopyright} The Royal Society of Chemistry and Owner Societies 2004.",

author = "Dan Elson and Jose Requejo-Isidro and Ian Munro and Fred Reavell and Jan Siegel and Klaus Suhling and Paul Tadrous and Richard Benninger and Peter Lanigan and James McGinty and Clifford Talbot and Bebhinn Treanor and Stephen Webb and Ann Sandison and Andrew Wallace and Dan Davis and John Lever and Mark Neil and David Philips and Gordon Stamp and Paul French",

year = "2004",

month = aug,

doi = "10.1039/b316456j",

language = "English",

volume = "3",

pages = "795--801",

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Elson, D, Requejo-Isidro, J, Munro, I, Reavell, F, Siegel, J, Suhling, K, Tadrous, P, Benninger, R, Lanigan, P, McGinty, J, Talbot, C, Treanor, B, Webb, S, Sandison, A, Wallace, A, Davis, D, Lever, J, Neil, M, Philips, D, Stamp, G & French, P 2004, 'Time-domain fluorescence lifetime imaging applied to biological tissue', Photochemical and Photobiological Sciences, vol. 3, no. 8, pp. 795-801. https://doi.org/10.1039/b316456j

TY - JOUR

T1 - Time-domain fluorescence lifetime imaging applied to biological tissue

AU - Elson, Dan

AU - Requejo-Isidro, Jose

AU - Munro, Ian

AU - Reavell, Fred

AU - Siegel, Jan

AU - Suhling, Klaus

AU - Tadrous, Paul

AU - Benninger, Richard

AU - Lanigan, Peter

AU - McGinty, James

AU - Talbot, Clifford

AU - Treanor, Bebhinn

AU - Webb, Stephen

AU - Sandison, Ann

AU - Wallace, Andrew

AU - Davis, Dan

AU - Lever, John

AU - Neil, Mark

AU - Philips, David

AU - Stamp, Gordon

AU - French, Paul

PY - 2004/8

Y1 - 2004/8

N2 - Fluorescence lifetime imaging (FLIM) is a functional imaging methodology that can provide information, not only concerning the localisation of specific fluorophores, but also about the local fluorophore environment. It may be implemented in scanning confocal or multi-photon microscopes, or in wide-field microscopes and endoscopes. When applied to tissue autofluorescence, it reveals intrinsic excellent contrast between different types and states of tissue. This article aims to review our recent progress in developing time-domain FLIM technology for microscopy and endoscopy and applying it to biological tissue. © The Royal Society of Chemistry and Owner Societies 2004.

AB - Fluorescence lifetime imaging (FLIM) is a functional imaging methodology that can provide information, not only concerning the localisation of specific fluorophores, but also about the local fluorophore environment. It may be implemented in scanning confocal or multi-photon microscopes, or in wide-field microscopes and endoscopes. When applied to tissue autofluorescence, it reveals intrinsic excellent contrast between different types and states of tissue. This article aims to review our recent progress in developing time-domain FLIM technology for microscopy and endoscopy and applying it to biological tissue. © The Royal Society of Chemistry and Owner Societies 2004.

U2 - 10.1039/b316456j

DO - 10.1039/b316456j

M3 - Article

C2 - 15295637

SN - 1474-9092

VL - 3

SP - 795

EP - 801

JO - Photochemical and Photobiological Sciences

JF - Photochemical and Photobiological Sciences

IS - 8

ER -

Time-domain fluorescence lifetime imaging applied to biological tissue

Abstract

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