Time-of-flight SIMS as a novel approach to unlocking the hypoxic properties of cancer

Emily G. Armitage; Helen L. Kotze; John S. Fletcher; Alex Henderson; Kaye J. Williams; Nicholas P. Lockyer; John C. Vickerman

doi:10.1002/sia.5050

Time-of-flight SIMS as a novel approach to unlocking the hypoxic properties of cancer

Emily G. Armitage, Helen L. Kotze, John S. Fletcher, Alex Henderson, Kaye J. Williams, Nicholas P. Lockyer, John C. Vickerman

Research output: Contribution to journal › Article › peer-review

113 Downloads (Pure)

Abstract

It is known that hypoxia-inducible factor 1 (HIF-1) activity results in the coordinated up-regulation of a large number of proteins that facilitate cell survival in tumours; however, the effect of HIF-1 on cancer metabolism is less well characterised. With knowledge of the specific effect of HIF-1 on cancer metabolism, biomarkers could be identified for which new drugs could be targeted. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) offers the potential to analyse intact cells in situ and has a mass spectral coverage that is applicable to metabolic profiling. It has been used to analyse the effects of HIF-1 on multicellular tumour models. Multicellular tumour spheroids (MTSs) have been cultured from human colon carcinoma cells with and without the expression of HIF-1, and the surface of the cross sections of each MTS has been analysed. Because metabolic profiling is an emerging field in ToF-SIMS, there is a requirement to determine which metabolites can be detected using this technique and which of those can be identified in complex mixtures within biological samples. For this, a selection of metabolites have been analysed, and the ToF-SIMS standard spectra acquired have been used to localise metabolites in MTS sections. The comparison of metabolic profiles of MTSs with and without the expression of HIF-1 has elucidated potential biomarkers for tumour survival in hypoxia, some of which may be HIF-1 regulated. Copyright © 2012 John Wiley & Sons, Ltd. Copyright © 2012 John Wiley & Sons, Ltd.

Original language	English
Pages (from-to)	282-285
Number of pages	3
Journal	Surface and Interface Analysis
Volume	45
Issue number	1
DOIs	https://doi.org/10.1002/sia.5050
Publication status	Published - Jan 2013

Keywords

cancer
HIF
hypoxia
metabolomics
PCA
ToF-SIMS

Research Beacons, Institutes and Platforms

Manchester Institute of Biotechnology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/sia.5050

POST-PEER-REVIEW-PUBLISHERS.PDFFinal published version, 216 KB

http://dx.doi.org/10.1002/sia.5050http://onlinelibrary.wiley.com/store/10.1002/sia.5050/asset/sia5050.pdf?v=1&t=h7w3qub0&s=1a7f8011eb25b5b06bdda431c4a7203b91466a16

Cite this

@article{1e8b3a188ee047c38a8354c6344af082,

title = "Time-of-flight SIMS as a novel approach to unlocking the hypoxic properties of cancer",

abstract = "It is known that hypoxia-inducible factor 1 (HIF-1) activity results in the coordinated up-regulation of a large number of proteins that facilitate cell survival in tumours; however, the effect of HIF-1 on cancer metabolism is less well characterised. With knowledge of the specific effect of HIF-1 on cancer metabolism, biomarkers could be identified for which new drugs could be targeted. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) offers the potential to analyse intact cells in situ and has a mass spectral coverage that is applicable to metabolic profiling. It has been used to analyse the effects of HIF-1 on multicellular tumour models. Multicellular tumour spheroids (MTSs) have been cultured from human colon carcinoma cells with and without the expression of HIF-1, and the surface of the cross sections of each MTS has been analysed. Because metabolic profiling is an emerging field in ToF-SIMS, there is a requirement to determine which metabolites can be detected using this technique and which of those can be identified in complex mixtures within biological samples. For this, a selection of metabolites have been analysed, and the ToF-SIMS standard spectra acquired have been used to localise metabolites in MTS sections. The comparison of metabolic profiles of MTSs with and without the expression of HIF-1 has elucidated potential biomarkers for tumour survival in hypoxia, some of which may be HIF-1 regulated. Copyright {\textcopyright} 2012 John Wiley & Sons, Ltd. Copyright {\textcopyright} 2012 John Wiley & Sons, Ltd.",

keywords = "cancer, HIF, hypoxia, metabolomics, PCA, ToF-SIMS",

author = "Armitage, {Emily G.} and Kotze, {Helen L.} and Fletcher, {John S.} and Alex Henderson and Williams, {Kaye J.} and Lockyer, {Nicholas P.} and Vickerman, {John C.}",

year = "2013",

month = jan,

doi = "10.1002/sia.5050",

language = "English",

volume = "45",

pages = "282--285",

journal = "Surface and Interface Analysis",

issn = "0142-2421",

publisher = "John Wiley & Sons Ltd",

number = "1",

}

TY - JOUR

T1 - Time-of-flight SIMS as a novel approach to unlocking the hypoxic properties of cancer

AU - Armitage, Emily G.

AU - Kotze, Helen L.

AU - Fletcher, John S.

AU - Henderson, Alex

AU - Williams, Kaye J.

AU - Lockyer, Nicholas P.

AU - Vickerman, John C.

PY - 2013/1

Y1 - 2013/1

N2 - It is known that hypoxia-inducible factor 1 (HIF-1) activity results in the coordinated up-regulation of a large number of proteins that facilitate cell survival in tumours; however, the effect of HIF-1 on cancer metabolism is less well characterised. With knowledge of the specific effect of HIF-1 on cancer metabolism, biomarkers could be identified for which new drugs could be targeted. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) offers the potential to analyse intact cells in situ and has a mass spectral coverage that is applicable to metabolic profiling. It has been used to analyse the effects of HIF-1 on multicellular tumour models. Multicellular tumour spheroids (MTSs) have been cultured from human colon carcinoma cells with and without the expression of HIF-1, and the surface of the cross sections of each MTS has been analysed. Because metabolic profiling is an emerging field in ToF-SIMS, there is a requirement to determine which metabolites can be detected using this technique and which of those can be identified in complex mixtures within biological samples. For this, a selection of metabolites have been analysed, and the ToF-SIMS standard spectra acquired have been used to localise metabolites in MTS sections. The comparison of metabolic profiles of MTSs with and without the expression of HIF-1 has elucidated potential biomarkers for tumour survival in hypoxia, some of which may be HIF-1 regulated. Copyright © 2012 John Wiley & Sons, Ltd. Copyright © 2012 John Wiley & Sons, Ltd.

AB - It is known that hypoxia-inducible factor 1 (HIF-1) activity results in the coordinated up-regulation of a large number of proteins that facilitate cell survival in tumours; however, the effect of HIF-1 on cancer metabolism is less well characterised. With knowledge of the specific effect of HIF-1 on cancer metabolism, biomarkers could be identified for which new drugs could be targeted. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) offers the potential to analyse intact cells in situ and has a mass spectral coverage that is applicable to metabolic profiling. It has been used to analyse the effects of HIF-1 on multicellular tumour models. Multicellular tumour spheroids (MTSs) have been cultured from human colon carcinoma cells with and without the expression of HIF-1, and the surface of the cross sections of each MTS has been analysed. Because metabolic profiling is an emerging field in ToF-SIMS, there is a requirement to determine which metabolites can be detected using this technique and which of those can be identified in complex mixtures within biological samples. For this, a selection of metabolites have been analysed, and the ToF-SIMS standard spectra acquired have been used to localise metabolites in MTS sections. The comparison of metabolic profiles of MTSs with and without the expression of HIF-1 has elucidated potential biomarkers for tumour survival in hypoxia, some of which may be HIF-1 regulated. Copyright © 2012 John Wiley & Sons, Ltd. Copyright © 2012 John Wiley & Sons, Ltd.

KW - cancer

KW - HIF

KW - hypoxia

KW - metabolomics

KW - PCA

KW - ToF-SIMS

U2 - 10.1002/sia.5050

DO - 10.1002/sia.5050

M3 - Article

SN - 0142-2421

VL - 45

SP - 282

EP - 285

JO - Surface and Interface Analysis

JF - Surface and Interface Analysis

IS - 1

ER -

Time-of-flight SIMS as a novel approach to unlocking the hypoxic properties of cancer

Abstract

Keywords

Research Beacons, Institutes and Platforms

UN SDGs

Access to Document

Fingerprint

Cite this