Time-resolved fluorescence anisotropy imaging applied to live cells

Klaus Suhling; Jan Siegel; Peter M P Lanigan; Sandrine Lévêque-Fort; Stephen E D Webb; David Phillips; Daniel M. Davis; Paul M W French

Time-resolved fluorescence anisotropy imaging applied to live cells

Klaus Suhling, Jan Siegel, Peter M P Lanigan, Sandrine Lévêque-Fort, Stephen E D Webb, David Phillips, Daniel M. Davis, Paul M W French

Research output: Contribution to journal › Article › peer-review

Abstract

We have developed a wide-field time-resolved imaging system to image quantitatively both the fluorescence lifetime and the rotational correlation time of a fluorophore. Using a polarization-resolved imager, we simultaneously image orthogonal polarization components of the fluorescence emission onto a time-gated intensified CCD. We demonstrate imaging of solvent viscosity variations through the rotational correlation time of fluorescein in a multiwell plate and apply this technique to probe the microviscosity in live cells. © 2004 Optical Society of America.

Original language	English
Pages (from-to)	584-586
Number of pages	2
Journal	Optics Letters
Volume	29
Issue number	6
Publication status	Published - 15 Mar 2004

Cite this

@article{4276338616444b08990eb5ecd7c54593,

title = "Time-resolved fluorescence anisotropy imaging applied to live cells",

abstract = "We have developed a wide-field time-resolved imaging system to image quantitatively both the fluorescence lifetime and the rotational correlation time of a fluorophore. Using a polarization-resolved imager, we simultaneously image orthogonal polarization components of the fluorescence emission onto a time-gated intensified CCD. We demonstrate imaging of solvent viscosity variations through the rotational correlation time of fluorescein in a multiwell plate and apply this technique to probe the microviscosity in live cells. {\textcopyright} 2004 Optical Society of America.",

author = "Klaus Suhling and Jan Siegel and Lanigan, {Peter M P} and Sandrine L{\'e}v{\^e}que-Fort and Webb, {Stephen E D} and David Phillips and Davis, {Daniel M.} and French, {Paul M W}",

year = "2004",

month = mar,

day = "15",

language = "English",

volume = "29",

pages = "584--586",

journal = "Optics Letters",

issn = "0146-9592",

publisher = "Optical Society of America",

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TY - JOUR

T1 - Time-resolved fluorescence anisotropy imaging applied to live cells

AU - Suhling, Klaus

AU - Siegel, Jan

AU - Lanigan, Peter M P

AU - Lévêque-Fort, Sandrine

AU - Webb, Stephen E D

AU - Phillips, David

AU - Davis, Daniel M.

AU - French, Paul M W

PY - 2004/3/15

Y1 - 2004/3/15

N2 - We have developed a wide-field time-resolved imaging system to image quantitatively both the fluorescence lifetime and the rotational correlation time of a fluorophore. Using a polarization-resolved imager, we simultaneously image orthogonal polarization components of the fluorescence emission onto a time-gated intensified CCD. We demonstrate imaging of solvent viscosity variations through the rotational correlation time of fluorescein in a multiwell plate and apply this technique to probe the microviscosity in live cells. © 2004 Optical Society of America.

AB - We have developed a wide-field time-resolved imaging system to image quantitatively both the fluorescence lifetime and the rotational correlation time of a fluorophore. Using a polarization-resolved imager, we simultaneously image orthogonal polarization components of the fluorescence emission onto a time-gated intensified CCD. We demonstrate imaging of solvent viscosity variations through the rotational correlation time of fluorescein in a multiwell plate and apply this technique to probe the microviscosity in live cells. © 2004 Optical Society of America.

M3 - Article

C2 - 15035478

SN - 0146-9592

VL - 29

SP - 584

EP - 586

JO - Optics Letters

JF - Optics Letters

IS - 6

ER -

Time-resolved fluorescence anisotropy imaging applied to live cells

Abstract

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