Timekeeping in the hindbrain: a multi-oscillatory circadian centre in the mouse dorsal vagal complex

Lukasz Chrobok, Rebecca C. Northeast, Jihwan Myung, Peter S. Cunningham, Cheryl Petit, Hugh D. Piggins

Research output: Contribution to journalArticlepeer-review


Metabolic and cardiovascular processes controlled by the hindbrain exhibit 24 h rhythms, but the extent to which the hindbrain possesses endogenous circadian timekeeping is unresolved. Here we provide compelling evidence that genetic, neuronal, and vascular activities of the brainstem’s dorsal vagal complex are subject to intrinsic circadian control with a crucial role for the connection between its components in regulating their rhythmic properties. Robust 24 h variation in clock gene expression in vivo and neuronal firing ex vivo were observed in the area postrema (AP) and nucleus of the solitary tract (NTS), together with enhanced nocturnal responsiveness to metabolic cues. Unexpectedly, we also find functional and molecular evidence for increased penetration of blood borne molecules into the NTS at night. Our findings reveal that the hindbrain houses a local network complex of neuronal and non-neuronal autonomous circadian oscillators, with clear implications for understanding local temporal control of physiology in the brainstem.
Original languageEnglish
JournalCommunications Biology
Issue number1
Early online date8 May 2020
Publication statusPublished - 1 Dec 2020


  • area postrema
  • circadian clock
  • Evans Blue
  • hindbrain
  • multi-electrode array
  • nucleus of the solitary tract
  • PER2::LUC


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