ToF-SIMS as a tool for metabolic profiling small biomolecules in cancer systems

Helen L. Kotze; Emily G. Armitage; John S. Fletcher; Alex Henderson; Kaye J. Williams; Nicholas P. Lockyer; John C. Vickerman

ToF-SIMS as a tool for metabolic profiling small biomolecules in cancer systems

Helen L. Kotze, Emily G. Armitage, John S. Fletcher, Alex Henderson, Kaye J. Williams, Nicholas P. Lockyer, John C. Vickerman

Research output: Chapter in Book/Conference proceeding › Conference contribution › peer-review

Abstract

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is emerging as a tool for studying the metabolism of disease. ToF-SIMS enables chemical specificity in addition to high spatial resolution imaging of biological samples from cells to tissue. Here, ToF-SIMS has been used to investigate the metabolic regulation of hypoxia-induced chemoresistance to doxorubicin treatment using multicellular tumour spheroids. Imaging principal component analysis (PCA) was used as a tool to identify the regions of chemistry present within the image that differ as a result of drug treatment. A series of metabolite ToF-SIMS spectra were acquired, which were used to identify quasi-molecular ions and fragments correlated to the PCA loading plots. Metabolite patterns have been identified as potential biomarkers of hypoxia-induced chemoresistance

Original language	English
Title of host publication	Surface and Interface Analysis
Publisher	John Wiley & Sons Ltd
Publication status	Published - 20 Jun 2012
Event	International Conference on Secondary Ion Mass Spectrometry - Lake Garda, Italy Duration: 1 Jan 1824 → …

Conference

Conference	International Conference on Secondary Ion Mass Spectrometry
City	Lake Garda, Italy
Period	1/01/24 → …

Keywords

metabolomics; ToF-SIMS; hypoxia; cancer; PCA; doxorubicin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

@inproceedings{374c0752cb4d416aaf36831af95391ae,

title = "ToF-SIMS as a tool for metabolic profiling small biomolecules in cancer systems",

abstract = "Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is emerging as a tool for studying the metabolism of disease. ToF-SIMS enables chemical specificity in addition to high spatial resolution imaging of biological samples from cells to tissue. Here, ToF-SIMS has been used to investigate the metabolic regulation of hypoxia-induced chemoresistance to doxorubicin treatment using multicellular tumour spheroids. Imaging principal component analysis (PCA) was used as a tool to identify the regions of chemistry present within the image that differ as a result of drug treatment. A series of metabolite ToF-SIMS spectra were acquired, which were used to identify quasi-molecular ions and fragments correlated to the PCA loading plots. Metabolite patterns have been identified as potential biomarkers of hypoxia-induced chemoresistance",

keywords = "metabolomics; ToF-SIMS; hypoxia; cancer; PCA; doxorubicin",

author = "Kotze, {Helen L.} and Armitage, {Emily G.} and Fletcher, {John S.} and Alex Henderson and Williams, {Kaye J.} and Lockyer, {Nicholas P.} and Vickerman, {John C.}",

year = "2012",

month = jun,

day = "20",

language = "English",

booktitle = "Surface and Interface Analysis",

publisher = "John Wiley & Sons Ltd",

address = "United Kingdom",

note = "International Conference on Secondary Ion Mass Spectrometry ; Conference date: 01-01-1824",

}

TY - GEN

T1 - ToF-SIMS as a tool for metabolic profiling small biomolecules in cancer systems

AU - Kotze, Helen L.

AU - Armitage, Emily G.

AU - Fletcher, John S.

AU - Henderson, Alex

AU - Williams, Kaye J.

AU - Lockyer, Nicholas P.

AU - Vickerman, John C.

PY - 2012/6/20

Y1 - 2012/6/20

N2 - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is emerging as a tool for studying the metabolism of disease. ToF-SIMS enables chemical specificity in addition to high spatial resolution imaging of biological samples from cells to tissue. Here, ToF-SIMS has been used to investigate the metabolic regulation of hypoxia-induced chemoresistance to doxorubicin treatment using multicellular tumour spheroids. Imaging principal component analysis (PCA) was used as a tool to identify the regions of chemistry present within the image that differ as a result of drug treatment. A series of metabolite ToF-SIMS spectra were acquired, which were used to identify quasi-molecular ions and fragments correlated to the PCA loading plots. Metabolite patterns have been identified as potential biomarkers of hypoxia-induced chemoresistance

AB - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is emerging as a tool for studying the metabolism of disease. ToF-SIMS enables chemical specificity in addition to high spatial resolution imaging of biological samples from cells to tissue. Here, ToF-SIMS has been used to investigate the metabolic regulation of hypoxia-induced chemoresistance to doxorubicin treatment using multicellular tumour spheroids. Imaging principal component analysis (PCA) was used as a tool to identify the regions of chemistry present within the image that differ as a result of drug treatment. A series of metabolite ToF-SIMS spectra were acquired, which were used to identify quasi-molecular ions and fragments correlated to the PCA loading plots. Metabolite patterns have been identified as potential biomarkers of hypoxia-induced chemoresistance

KW - metabolomics; ToF-SIMS; hypoxia; cancer; PCA; doxorubicin

M3 - Conference contribution

BT - Surface and Interface Analysis

PB - John Wiley & Sons Ltd

T2 - International Conference on Secondary Ion Mass Spectrometry

Y2 - 1 January 1824

ER -

ToF-SIMS as a tool for metabolic profiling small biomolecules in cancer systems

Abstract

Conference

Keywords

UN SDGs

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