ToF-SIMS as a tool for metabolic profiling small biomolecules in cancer systems

Helen L. Kotze, Emily G. Armitage, John S. Fletcher, Alex Henderson, Kaye J. Williams, Nicholas P. Lockyer, John C. Vickerman

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    Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is emerging as a tool for studying the metabolism of disease. ToF-SIMS enables chemical specificity in addition to high spatial resolution imaging of biological samples from cells to tissue. Here, ToF-SIMS has been used to investigate the metabolic regulation of hypoxia-induced chemoresistance to doxorubicin treatment using multicellular tumour spheroids. Imaging principal component analysis (PCA) was used as a tool to identify the regions of chemistry present within the image that differ as a result of drug treatment. A series of metabolite ToF-SIMS spectra were acquired, which were used to identify quasi-molecular ions and fragments correlated to the PCA loading plots. Metabolite patterns have been identified as potential biomarkers of hypoxia-induced chemoresistance. Copyright © 2012 John Wiley & Sons, Ltd. Copyright © 2012 John Wiley & Sons, Ltd.
    Original languageEnglish
    Pages (from-to)277-281
    Number of pages4
    JournalSurface and Interface Analysis
    Issue number1
    Publication statusPublished - Jan 2013


    • cancer
    • doxorubicin
    • hypoxia
    • metabolomics
    • PCA
    • ToF-SIMS

    Research Beacons, Institutes and Platforms

    • Manchester Institute of Biotechnology


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