ToF-SIMS as a tool for metabolic profiling small biomolecules in cancer systems

Helen L. Kotze; Emily G. Armitage; John S. Fletcher; Alex Henderson; Kaye J. Williams; Nicholas P. Lockyer; John C. Vickerman

doi:10.1002/sia.5055

ToF-SIMS as a tool for metabolic profiling small biomolecules in cancer systems

Helen L. Kotze, Emily G. Armitage, John S. Fletcher, Alex Henderson, Kaye J. Williams, Nicholas P. Lockyer, John C. Vickerman

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Abstract

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is emerging as a tool for studying the metabolism of disease. ToF-SIMS enables chemical specificity in addition to high spatial resolution imaging of biological samples from cells to tissue. Here, ToF-SIMS has been used to investigate the metabolic regulation of hypoxia-induced chemoresistance to doxorubicin treatment using multicellular tumour spheroids. Imaging principal component analysis (PCA) was used as a tool to identify the regions of chemistry present within the image that differ as a result of drug treatment. A series of metabolite ToF-SIMS spectra were acquired, which were used to identify quasi-molecular ions and fragments correlated to the PCA loading plots. Metabolite patterns have been identified as potential biomarkers of hypoxia-induced chemoresistance. Copyright © 2012 John Wiley & Sons, Ltd. Copyright © 2012 John Wiley & Sons, Ltd.

Original language	English
Pages (from-to)	277-281
Number of pages	4
Journal	Surface and Interface Analysis
Volume	45
Issue number	1
DOIs	https://doi.org/10.1002/sia.5055
Publication status	Published - Jan 2013

Keywords

cancer
doxorubicin
hypoxia
metabolomics
PCA
ToF-SIMS

Research Beacons, Institutes and Platforms

Manchester Institute of Biotechnology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Cite this

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title = "ToF-SIMS as a tool for metabolic profiling small biomolecules in cancer systems",

abstract = "Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is emerging as a tool for studying the metabolism of disease. ToF-SIMS enables chemical specificity in addition to high spatial resolution imaging of biological samples from cells to tissue. Here, ToF-SIMS has been used to investigate the metabolic regulation of hypoxia-induced chemoresistance to doxorubicin treatment using multicellular tumour spheroids. Imaging principal component analysis (PCA) was used as a tool to identify the regions of chemistry present within the image that differ as a result of drug treatment. A series of metabolite ToF-SIMS spectra were acquired, which were used to identify quasi-molecular ions and fragments correlated to the PCA loading plots. Metabolite patterns have been identified as potential biomarkers of hypoxia-induced chemoresistance. Copyright {\textcopyright} 2012 John Wiley & Sons, Ltd. Copyright {\textcopyright} 2012 John Wiley & Sons, Ltd.",

keywords = "cancer, doxorubicin, hypoxia, metabolomics, PCA, ToF-SIMS",

author = "Kotze, {Helen L.} and Armitage, {Emily G.} and Fletcher, {John S.} and Alex Henderson and Williams, {Kaye J.} and Lockyer, {Nicholas P.} and Vickerman, {John C.}",

year = "2013",

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doi = "10.1002/sia.5055",

language = "English",

volume = "45",

pages = "277--281",

journal = "Surface and Interface Analysis",

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T1 - ToF-SIMS as a tool for metabolic profiling small biomolecules in cancer systems

AU - Kotze, Helen L.

AU - Armitage, Emily G.

AU - Fletcher, John S.

AU - Henderson, Alex

AU - Williams, Kaye J.

AU - Lockyer, Nicholas P.

AU - Vickerman, John C.

PY - 2013/1

Y1 - 2013/1

N2 - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is emerging as a tool for studying the metabolism of disease. ToF-SIMS enables chemical specificity in addition to high spatial resolution imaging of biological samples from cells to tissue. Here, ToF-SIMS has been used to investigate the metabolic regulation of hypoxia-induced chemoresistance to doxorubicin treatment using multicellular tumour spheroids. Imaging principal component analysis (PCA) was used as a tool to identify the regions of chemistry present within the image that differ as a result of drug treatment. A series of metabolite ToF-SIMS spectra were acquired, which were used to identify quasi-molecular ions and fragments correlated to the PCA loading plots. Metabolite patterns have been identified as potential biomarkers of hypoxia-induced chemoresistance. Copyright © 2012 John Wiley & Sons, Ltd. Copyright © 2012 John Wiley & Sons, Ltd.

AB - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is emerging as a tool for studying the metabolism of disease. ToF-SIMS enables chemical specificity in addition to high spatial resolution imaging of biological samples from cells to tissue. Here, ToF-SIMS has been used to investigate the metabolic regulation of hypoxia-induced chemoresistance to doxorubicin treatment using multicellular tumour spheroids. Imaging principal component analysis (PCA) was used as a tool to identify the regions of chemistry present within the image that differ as a result of drug treatment. A series of metabolite ToF-SIMS spectra were acquired, which were used to identify quasi-molecular ions and fragments correlated to the PCA loading plots. Metabolite patterns have been identified as potential biomarkers of hypoxia-induced chemoresistance. Copyright © 2012 John Wiley & Sons, Ltd. Copyright © 2012 John Wiley & Sons, Ltd.

KW - cancer

KW - doxorubicin

KW - hypoxia

KW - metabolomics

KW - PCA

KW - ToF-SIMS

U2 - 10.1002/sia.5055

DO - 10.1002/sia.5055

M3 - Article

SN - 0142-2421

VL - 45

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EP - 281

JO - Surface and Interface Analysis

JF - Surface and Interface Analysis

IS - 1

ER -

ToF-SIMS as a tool for metabolic profiling small biomolecules in cancer systems

Abstract

Keywords

Research Beacons, Institutes and Platforms

UN SDGs

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