Abstract
In order to investigate the extent of genome rearrangements in laboratory and industrial yeast strains, a set of plasmids, containing ca. 300 bp fragments from highly conserved genes from S. cerevisiae, has been constructed. We chose three unique PCR products, each from a single gene, per chromosome: one from close to the centromere, and one from each chromosome end. Using these plasmids as probes to hybridize a Southern blot from a pulsed-field gel electrophoresis separation of the 16 yeast chromosomes, it is possible to identify large chromosomal rearrangements such as reciprocal translocations. Copyright © 2002 John Wiley & Sons, Ltd.
| Original language | English |
|---|---|
| Pages (from-to) | 441-448 |
| Number of pages | 7 |
| Journal | Yeast |
| Volume | 19 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 30 Mar 2002 |
Keywords
- Evolution
- Genome rearrangements
- Karyotypes
- Translocations
Research Beacons, Institutes and Platforms
- Manchester Institute of Biotechnology