Total nucleic acid analysis integrated on microfluidic devices

Philip Day, Lin Chen, Andreas Manz, Philip J R Day

    Research output: Contribution to journalArticlepeer-review


    The design and integration of microfluidic devices for on-chip amplification of nucleic acids from various biological samples has undergone extensive development. The actual benefit to the biological community is far from clear, with a growing, but limited, number of application successes in terms of a full on-chip integrated analysis. Several advances have been made, particularly with the integration of amplification and detection, where amplification is most often the polymerase chain reaction. Full integration including sample preparation remains a major obstacle for achieving a quantitative analysis. We review the recently described devices incorporating in vitro gene amplification and compare devices relative to each other and in terms of fully achieving a miniaturised total analysis system (μ-TAS). © The Royal Society of Chemistry.
    Original languageEnglish
    Pages (from-to)1413-1423
    Number of pages10
    JournalLab on a Chip: miniaturisation for chemistry, physics, biology, materials science and bioengineering
    Issue number11
    Publication statusPublished - 2007


    • analysis
    • Electrochemistry
    • Electrophoresis,Capillary
    • Immunoassay
    • instrumentation
    • Microfluidics
    • Nucleic Acid Hybridization
    • Nucleic Acids
    • Polymerase Chain Reaction


    Dive into the research topics of 'Total nucleic acid analysis integrated on microfluidic devices'. Together they form a unique fingerprint.

    Cite this