Toward a physical map of the genome of the nematode Caenorhabditis elegans.

Alan Coulson; John Sulston; Sydney Brenner; Jonathan Karn

Toward a physical map of the genome of the nematode Caenorhabditis elegans.

Alan Coulson, John Sulston, Sydney Brenner, Jonathan Karn

Research output: Contribution to journal › Article › peer-review

Abstract

A technique for digital characterization and comparison of DNA fragments, using restriction enzymes, is described. The technique is being applied to fragments from the nematode Caenorhabditis elegans (i) to facilitate cross-indexing of clones emanating from different laboratories and (ii) to construct a physical map of the genome. Eight hundred sixty clusters of clones, from 35 to 350 kilobases long and totaling about 60% of the genome, have been characterized.

Original language	English
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	83
Issue number	20
Publication status	Published - Oct 1986

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title = "Toward a physical map of the genome of the nematode Caenorhabditis elegans.",

abstract = "A technique for digital characterization and comparison of DNA fragments, using restriction enzymes, is described. The technique is being applied to fragments from the nematode Caenorhabditis elegans (i) to facilitate cross-indexing of clones emanating from different laboratories and (ii) to construct a physical map of the genome. Eight hundred sixty clusters of clones, from 35 to 350 kilobases long and totaling about 60% of the genome, have been characterized.",

author = "Alan Coulson and John Sulston and Sydney Brenner and Jonathan Karn",

year = "1986",

month = oct,

language = "English",

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journal = "Proceedings of the National Academy of Sciences of the United States of America",

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publisher = "National Academy of Sciences",

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T1 - Toward a physical map of the genome of the nematode Caenorhabditis elegans.

AU - Coulson, Alan

AU - Sulston, John

AU - Brenner, Sydney

AU - Karn, Jonathan

PY - 1986/10

Y1 - 1986/10

N2 - A technique for digital characterization and comparison of DNA fragments, using restriction enzymes, is described. The technique is being applied to fragments from the nematode Caenorhabditis elegans (i) to facilitate cross-indexing of clones emanating from different laboratories and (ii) to construct a physical map of the genome. Eight hundred sixty clusters of clones, from 35 to 350 kilobases long and totaling about 60% of the genome, have been characterized.

AB - A technique for digital characterization and comparison of DNA fragments, using restriction enzymes, is described. The technique is being applied to fragments from the nematode Caenorhabditis elegans (i) to facilitate cross-indexing of clones emanating from different laboratories and (ii) to construct a physical map of the genome. Eight hundred sixty clusters of clones, from 35 to 350 kilobases long and totaling about 60% of the genome, have been characterized.

M3 - Article

C2 - 16593771

SN - 1091-6490

VL - 83

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 20

ER -

Toward a physical map of the genome of the nematode Caenorhabditis elegans.

Abstract

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