Transcription factor XBP-1 is expressed during osteoblast differentiation and is transcriptionally regulated by parathyroid hormone (PTH)

A. Zambelli; E. Mongiardini; S. N. Villegas; N. G. Carri; R. P. Boot-Handford; G. A. Wallis

doi:10.1016/j.cellbi.2005.03.018

Transcription factor XBP-1 is expressed during osteoblast differentiation and is transcriptionally regulated by parathyroid hormone (PTH)

A. Zambelli, E. Mongiardini, S. N. Villegas, N. G. Carri, R. P. Boot-Handford, G. A. Wallis

Research output: Contribution to journal › Article › peer-review

Abstract

X-box-binding protein 1 (XBP-1) is a basic-region leucine zipper protein in the cyclic AMP response element binding protein/activating transcription factor (CREB/ATF) family of transcription factors involved in different cell-differentiation processes. We have investigated the expression of XBP-1 in differentiating MC3T3-E1 osteoblastic cells. Cultures were treated with ascorbic acid (AA) and β-glycerophosphate (BGP) to induce differentiation. Under these conditions, the basal transcription of xbp-1 increases at day 2 following induction, peaks at day 5 and decreases thereafter. This result showed that xbp-1 gene is differentially expressed during MC3T3-E1 cell differentiation. Detection of XBP-1 by immunofluorescence at days 0 (control culture without AA and BGP), 8 and 21 showed that the protein has a major cytoplasmic perinuclear location. In addition, xbp-1 is transcriptionally upregulated by parathyroid hormone within 2.5 h of treatment and decreases thereafter. © 2005 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	647-653
Number of pages	6
Journal	Cell Biology International
Volume	29
Issue number	8
DOIs	https://doi.org/10.1016/j.cellbi.2005.03.018
Publication status	Published - Aug 2005

Keywords

Osteoblast differentiation
PTH
XBP-1

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10.1016/j.cellbi.2005.03.018

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@article{e8dd7ba7d01c4b038d8d24066e643636,

title = "Transcription factor XBP-1 is expressed during osteoblast differentiation and is transcriptionally regulated by parathyroid hormone (PTH)",

abstract = "X-box-binding protein 1 (XBP-1) is a basic-region leucine zipper protein in the cyclic AMP response element binding protein/activating transcription factor (CREB/ATF) family of transcription factors involved in different cell-differentiation processes. We have investigated the expression of XBP-1 in differentiating MC3T3-E1 osteoblastic cells. Cultures were treated with ascorbic acid (AA) and β-glycerophosphate (BGP) to induce differentiation. Under these conditions, the basal transcription of xbp-1 increases at day 2 following induction, peaks at day 5 and decreases thereafter. This result showed that xbp-1 gene is differentially expressed during MC3T3-E1 cell differentiation. Detection of XBP-1 by immunofluorescence at days 0 (control culture without AA and BGP), 8 and 21 showed that the protein has a major cytoplasmic perinuclear location. In addition, xbp-1 is transcriptionally upregulated by parathyroid hormone within 2.5 h of treatment and decreases thereafter. {\textcopyright} 2005 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.",

keywords = "Osteoblast differentiation, PTH, XBP-1",

author = "A. Zambelli and E. Mongiardini and Villegas, {S. N.} and Carri, {N. G.} and Boot-Handford, {R. P.} and Wallis, {G. A.}",

year = "2005",

month = aug,

doi = "10.1016/j.cellbi.2005.03.018",

language = "English",

volume = "29",

pages = "647--653",

journal = "Cell Biology International",

issn = "1065-6995",

publisher = "Portland Press Ltd",

number = "8",

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TY - JOUR

T1 - Transcription factor XBP-1 is expressed during osteoblast differentiation and is transcriptionally regulated by parathyroid hormone (PTH)

AU - Zambelli, A.

AU - Mongiardini, E.

AU - Villegas, S. N.

AU - Carri, N. G.

AU - Boot-Handford, R. P.

AU - Wallis, G. A.

PY - 2005/8

Y1 - 2005/8

N2 - X-box-binding protein 1 (XBP-1) is a basic-region leucine zipper protein in the cyclic AMP response element binding protein/activating transcription factor (CREB/ATF) family of transcription factors involved in different cell-differentiation processes. We have investigated the expression of XBP-1 in differentiating MC3T3-E1 osteoblastic cells. Cultures were treated with ascorbic acid (AA) and β-glycerophosphate (BGP) to induce differentiation. Under these conditions, the basal transcription of xbp-1 increases at day 2 following induction, peaks at day 5 and decreases thereafter. This result showed that xbp-1 gene is differentially expressed during MC3T3-E1 cell differentiation. Detection of XBP-1 by immunofluorescence at days 0 (control culture without AA and BGP), 8 and 21 showed that the protein has a major cytoplasmic perinuclear location. In addition, xbp-1 is transcriptionally upregulated by parathyroid hormone within 2.5 h of treatment and decreases thereafter. © 2005 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

AB - X-box-binding protein 1 (XBP-1) is a basic-region leucine zipper protein in the cyclic AMP response element binding protein/activating transcription factor (CREB/ATF) family of transcription factors involved in different cell-differentiation processes. We have investigated the expression of XBP-1 in differentiating MC3T3-E1 osteoblastic cells. Cultures were treated with ascorbic acid (AA) and β-glycerophosphate (BGP) to induce differentiation. Under these conditions, the basal transcription of xbp-1 increases at day 2 following induction, peaks at day 5 and decreases thereafter. This result showed that xbp-1 gene is differentially expressed during MC3T3-E1 cell differentiation. Detection of XBP-1 by immunofluorescence at days 0 (control culture without AA and BGP), 8 and 21 showed that the protein has a major cytoplasmic perinuclear location. In addition, xbp-1 is transcriptionally upregulated by parathyroid hormone within 2.5 h of treatment and decreases thereafter. © 2005 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

KW - Osteoblast differentiation

KW - PTH

KW - XBP-1

U2 - 10.1016/j.cellbi.2005.03.018

DO - 10.1016/j.cellbi.2005.03.018

M3 - Article

SN - 1065-6995

VL - 29

SP - 647

EP - 653

JO - Cell Biology International

JF - Cell Biology International

IS - 8

ER -

Transcription factor XBP-1 is expressed during osteoblast differentiation and is transcriptionally regulated by parathyroid hormone (PTH)

Abstract

Keywords

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