Transcriptome analysis of human ageing in male skin shows mid-life period of variability and central role of NF-κB

Daniel J. Haustead, Andrew Stevenson, Vishal Saxena, Fiona Marriage, Martin Firth, Robyn Silla, Lisa W Martin, Katharine F. Adcroft, Suzanne M. Rea, Philip Day, Phillip Melton, Fiona M. Wood, Mark W. Fear

    Research output: Contribution to journalArticlepeer-review


    Age is well-known to be a significant factor in both disease pathology and response to treatment, yet the molecular changes that occur with age in humans remain ill-defined. Here, using transcriptome profiling of healthy human male skin, we demonstrate that there is a period of significantly elevated, transcriptome-wide expression changes occurring predominantly in middle age. Both pre and post this period, the transcriptome appears to undergo much smaller, linear changes with increasing age. Functional analysis of the transient changes in middle age suggest a period of heightened metabolic activity and cellular damage associated with NF-kappa-B and TNF signaling pathways. Through meta-analysis we also show the presence of global, tissue independent linear transcriptome changes with age which appear to be regulated by NF-kappa-B. These results suggest that aging in human skin is associated with a critical mid-life period with widespread transcriptome changes, both preceded and proceeded by a relatively steady rate of linear change in the transcriptome. The data provides insight into molecular changes associated with normal aging and will help to better understand the increasingly important pathological changes associated with aging.

    Original languageEnglish
    Article number26846
    JournalScientific Reports
    Publication statusPublished - 27 May 2016


    Dive into the research topics of 'Transcriptome analysis of human ageing in male skin shows mid-life period of variability and central role of NF-κB'. Together they form a unique fingerprint.

    Cite this