Transcriptome and proteome analysis of osteocytes treated with nitrogen-containing bisphosphonates

Nicoletta Bivi, Jessica Z. Bereszczak, Milena Romanello, Leo A H Zeef, Daniela Delneri, Franco Quadrifoglio, Luigi Moro, Francesco L. Brancia, Gianluca Tell

    Research output: Contribution to journalArticlepeer-review


    We combined high-throughput screening of differential mRNAs with mass spectrometric characterization of proteins obtained from osteocytes untreated and treated with Risedronate. Microarray analysis revealed, upon treatment, a marked upregulation of messengers encoding zinc-proteins. MS analysis identified 84 proteins in the osteocytes proteome map. Risedronate affected the expression of 10 proteins, associated with cytoskeleton, stress-response and metabolism. Data validated using gel imaging in combination with the GLaD post digestion isotopic labeling method provide the molecular basis for understanding the role of bisphosphonates as antiapoptotic drugs for osteocytes. © 2009 American Chemical Society.
    Original languageEnglish
    Pages (from-to)1131-1142
    Number of pages11
    JournalJournal of Proteome Research
    Issue number3
    Publication statusPublished - 6 Mar 2009


    • Bisphosphonates
    • Differential proteomics
    • Gene expression
    • Glad
    • Isotopic
    • Labelling
    • Osteocytes


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