TY - JOUR
T1 - Transcriptomic analysis of the sulfate starvation response of Pseudomonas aeruginosa
AU - Kertesz, Michael
AU - Tralau, Tewes
AU - Vuilleumier, Stéphane
AU - Thibault, Christelle
AU - Campbell, Barry J.
AU - Hart, C. Anthony
AU - Kertesz, Michael A.
PY - 2007/10
Y1 - 2007/10
N2 - Pseudomonas aeruginosa is an opportunistic pathogen that causes a number of infections in humans, but is best known for its association with cystic fibrosis. It is able to use a wide range of sulfur compounds as sources of sulfur for growth. Gene expression in response to changes in sulfur supply was studied in P. aeruginosa E601, a cystic fibrosis isolate that displays mucin sulfatase activity, and in P. aeruginosa PAO1. A large family of genes was found to be upregulated by sulfate limitation in both isolates, encoding sulfatases and sulfonatases, transport systems, oxidative stress proteins, and a sulfate-regulated TonB/ExbBD complex. These genes were localized in five distinct islands on the genome and encoded proteins with a significantly reduced content of cysteine and methionine. Growth of P. aeruginosa E601 with mucin as the sulfur source led not only to a sulfate starvation response but also to induction of genes involved with type III secretion systems. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
AB - Pseudomonas aeruginosa is an opportunistic pathogen that causes a number of infections in humans, but is best known for its association with cystic fibrosis. It is able to use a wide range of sulfur compounds as sources of sulfur for growth. Gene expression in response to changes in sulfur supply was studied in P. aeruginosa E601, a cystic fibrosis isolate that displays mucin sulfatase activity, and in P. aeruginosa PAO1. A large family of genes was found to be upregulated by sulfate limitation in both isolates, encoding sulfatases and sulfonatases, transport systems, oxidative stress proteins, and a sulfate-regulated TonB/ExbBD complex. These genes were localized in five distinct islands on the genome and encoded proteins with a significantly reduced content of cysteine and methionine. Growth of P. aeruginosa E601 with mucin as the sulfur source led not only to a sulfate starvation response but also to induction of genes involved with type III secretion systems. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
U2 - 10.1128/JB.00889-07
DO - 10.1128/JB.00889-07
M3 - Article
SN - 0021-9193
VL - 189
SP - 6743
EP - 6750
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 19
ER -
