Transcriptomic Profiling of Human Choriodecidua During Term Labor: Inflammation as a Key Driver of Labor.

Gillian L Stephen; Sylvia Lui; Sarah A Hamilton; Clare L Tower; Lynda K Harris; Adam Stevens; Rebecca L Jones

doi:10.1111/aji.12328

Transcriptomic Profiling of Human Choriodecidua During Term Labor: Inflammation as a Key Driver of Labor.

Gillian L Stephen, Sylvia Lui, Sarah A Hamilton, Clare L Tower, Lynda K Harris, Adam Stevens, Rebecca L Jones

Research output: Contribution to journal › Article › peer-review

Abstract

PROBLEM: Inflammation is a driver of labor in myometrium and cervix; however, the involvement of decidua is poorly defined. We have reported decidual leukocyte infiltration prior to and during labor; the regulators of these inflammatory processes are unknown. METHOD OF STUDY: Choriodecidua RNA obtained after term labor or elective cesarean delivery was applied to Affymetrix GeneChips. Pathway analysis and gene validation were performed. RESULTS: Extensive inflammatory activation was identified in choriodecidua following labor, predominantly upregulation of genes regulating leukocyte trafficking and cytokine signalling. Genes governing cell fate, tissue remodelling, and translation were also altered. Upregulation of candidate genes (ICAM1, CXCR4, CD44, TLR4, SOCS3, BCL2A, and IDO) was confirmed. NFκB, STAT1&3, HMGB1, and miRNA-21, miRNA-46, miRNA-141, and miRNA-200 were predicted upstream regulators. CONCLUSION: This study confirms inflammatory processes are major players in labor events in choriodecidua, as in other gestational tissues. Suppressing uterine inflammation is likely to be critical for arresting premature labor.

Original language	English
Pages (from-to)	36-55
Number of pages	19
Journal	American journal of reproductive immunology (New York, N.Y. : 1989)
Volume	73
Issue number	1
DOIs	https://doi.org/10.1111/aji.12328
Publication status	Published - 4 Oct 2014

Keywords

Chemokines
choriodecidua
miRNAs
pathway analysis
transcription factors

Access to Document

10.1111/aji.12328

Cite this

@article{95cf28db641449d98473519251025723,

title = "Transcriptomic Profiling of Human Choriodecidua During Term Labor: Inflammation as a Key Driver of Labor.",

abstract = "PROBLEM: Inflammation is a driver of labor in myometrium and cervix; however, the involvement of decidua is poorly defined. We have reported decidual leukocyte infiltration prior to and during labor; the regulators of these inflammatory processes are unknown. METHOD OF STUDY: Choriodecidua RNA obtained after term labor or elective cesarean delivery was applied to Affymetrix GeneChips. Pathway analysis and gene validation were performed. RESULTS: Extensive inflammatory activation was identified in choriodecidua following labor, predominantly upregulation of genes regulating leukocyte trafficking and cytokine signalling. Genes governing cell fate, tissue remodelling, and translation were also altered. Upregulation of candidate genes (ICAM1, CXCR4, CD44, TLR4, SOCS3, BCL2A, and IDO) was confirmed. NFκB, STAT1&3, HMGB1, and miRNA-21, miRNA-46, miRNA-141, and miRNA-200 were predicted upstream regulators. CONCLUSION: This study confirms inflammatory processes are major players in labor events in choriodecidua, as in other gestational tissues. Suppressing uterine inflammation is likely to be critical for arresting premature labor.",

keywords = "Chemokines, choriodecidua, miRNAs, pathway analysis, transcription factors",

author = "Stephen, {Gillian L} and Sylvia Lui and Hamilton, {Sarah A} and Tower, {Clare L} and Harris, {Lynda K} and Adam Stevens and Jones, {Rebecca L}",

year = "2014",

month = oct,

day = "4",

doi = "10.1111/aji.12328",

language = "English",

volume = "73",

pages = "36--55",

journal = "American journal of reproductive immunology (New York, N.Y. : 1989)",

issn = "1600-0897",

publisher = "John Wiley & Sons Ltd",

number = "1",

}

TY - JOUR

T1 - Transcriptomic Profiling of Human Choriodecidua During Term Labor: Inflammation as a Key Driver of Labor.

AU - Stephen, Gillian L

AU - Lui, Sylvia

AU - Hamilton, Sarah A

AU - Tower, Clare L

AU - Harris, Lynda K

AU - Stevens, Adam

AU - Jones, Rebecca L

PY - 2014/10/4

Y1 - 2014/10/4

N2 - PROBLEM: Inflammation is a driver of labor in myometrium and cervix; however, the involvement of decidua is poorly defined. We have reported decidual leukocyte infiltration prior to and during labor; the regulators of these inflammatory processes are unknown. METHOD OF STUDY: Choriodecidua RNA obtained after term labor or elective cesarean delivery was applied to Affymetrix GeneChips. Pathway analysis and gene validation were performed. RESULTS: Extensive inflammatory activation was identified in choriodecidua following labor, predominantly upregulation of genes regulating leukocyte trafficking and cytokine signalling. Genes governing cell fate, tissue remodelling, and translation were also altered. Upregulation of candidate genes (ICAM1, CXCR4, CD44, TLR4, SOCS3, BCL2A, and IDO) was confirmed. NFκB, STAT1&3, HMGB1, and miRNA-21, miRNA-46, miRNA-141, and miRNA-200 were predicted upstream regulators. CONCLUSION: This study confirms inflammatory processes are major players in labor events in choriodecidua, as in other gestational tissues. Suppressing uterine inflammation is likely to be critical for arresting premature labor.

AB - PROBLEM: Inflammation is a driver of labor in myometrium and cervix; however, the involvement of decidua is poorly defined. We have reported decidual leukocyte infiltration prior to and during labor; the regulators of these inflammatory processes are unknown. METHOD OF STUDY: Choriodecidua RNA obtained after term labor or elective cesarean delivery was applied to Affymetrix GeneChips. Pathway analysis and gene validation were performed. RESULTS: Extensive inflammatory activation was identified in choriodecidua following labor, predominantly upregulation of genes regulating leukocyte trafficking and cytokine signalling. Genes governing cell fate, tissue remodelling, and translation were also altered. Upregulation of candidate genes (ICAM1, CXCR4, CD44, TLR4, SOCS3, BCL2A, and IDO) was confirmed. NFκB, STAT1&3, HMGB1, and miRNA-21, miRNA-46, miRNA-141, and miRNA-200 were predicted upstream regulators. CONCLUSION: This study confirms inflammatory processes are major players in labor events in choriodecidua, as in other gestational tissues. Suppressing uterine inflammation is likely to be critical for arresting premature labor.

KW - Chemokines

KW - choriodecidua

KW - miRNAs

KW - pathway analysis

KW - transcription factors

U2 - 10.1111/aji.12328

DO - 10.1111/aji.12328

M3 - Article

C2 - 25283845

SN - 1600-0897

VL - 73

SP - 36

EP - 55

JO - American journal of reproductive immunology (New York, N.Y. : 1989)

JF - American journal of reproductive immunology (New York, N.Y. : 1989)

IS - 1

ER -

Transcriptomic Profiling of Human Choriodecidua During Term Labor: Inflammation as a Key Driver of Labor.

Abstract

Keywords

Access to Document

Fingerprint

Cite this