Transforming growth factor-β (TGFβ) receptors I/II differentially regulate TGFβ1 and IGF-binding protein-3 mitogenic effects in the human placenta

Karen Forbes; Benoit Souquet; Rebecca Garside; John D. Aplin; Melissa Westwood

doi:10.1210/en.2009-0896

Transforming growth factor-β (TGFβ) receptors I/II differentially regulate TGFβ1 and IGF-binding protein-3 mitogenic effects in the human placenta

Karen Forbes, Benoit Souquet, Rebecca Garside, John D. Aplin, Melissa Westwood

Research output: Contribution to journal › Article › peer-review

Abstract

Maternal IGFs regulate cytotrophoblast proliferation and, thereby, placental growth and function.IGF bioavailability is controlled by IGF-binding proteins (IGFBPs); in placenta, IGFBP-3 is particularly abundant. In other systems, IGFBP-3 can regulate cellular events independently of IGFs; these effects are thought to be mediated by TGFβreceptors (TβR).We have examined IGFBP-3 regulation of IGF-dependent and -independent cytotrophoblast proliferation in first-trimester placental explants and the role of TβRII in mediating these effects. In the presence of IGFBP-3 (50 nM), IGF-induced (10 nM) proliferation (monitored by immunohistochemical analysis of Ki67 expression and bromodeoxyuridine incorporation) was significantly reduced (P

Original language	English
Pages (from-to)	1723-1731
Number of pages	8
Journal	Endocrinology
Volume	151
Issue number	4
DOIs	https://doi.org/10.1210/en.2009-0896
Publication status	Published - Apr 2010

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http://endo.endojournals.org/cgi/reprint/151/4/1723

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title = "Transforming growth factor-β (TGFβ) receptors I/II differentially regulate TGFβ1 and IGF-binding protein-3 mitogenic effects in the human placenta",

abstract = "Maternal IGFs regulate cytotrophoblast proliferation and, thereby, placental growth and function.IGF bioavailability is controlled by IGF-binding proteins (IGFBPs); in placenta, IGFBP-3 is particularly abundant. In other systems, IGFBP-3 can regulate cellular events independently of IGFs; these effects are thought to be mediated by TGFβreceptors (TβR).We have examined IGFBP-3 regulation of IGF-dependent and -independent cytotrophoblast proliferation in first-trimester placental explants and the role of TβRII in mediating these effects. In the presence of IGFBP-3 (50 nM), IGF-induced (10 nM) proliferation (monitored by immunohistochemical analysis of Ki67 expression and bromodeoxyuridine incorporation) was significantly reduced (P",

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AU - Souquet, Benoit

AU - Garside, Rebecca

AU - Aplin, John D.

AU - Westwood, Melissa

PY - 2010/4

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AB - Maternal IGFs regulate cytotrophoblast proliferation and, thereby, placental growth and function.IGF bioavailability is controlled by IGF-binding proteins (IGFBPs); in placenta, IGFBP-3 is particularly abundant. In other systems, IGFBP-3 can regulate cellular events independently of IGFs; these effects are thought to be mediated by TGFβreceptors (TβR).We have examined IGFBP-3 regulation of IGF-dependent and -independent cytotrophoblast proliferation in first-trimester placental explants and the role of TβRII in mediating these effects. In the presence of IGFBP-3 (50 nM), IGF-induced (10 nM) proliferation (monitored by immunohistochemical analysis of Ki67 expression and bromodeoxyuridine incorporation) was significantly reduced (P

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JO - Endocrinology

JF - Endocrinology

IS - 4

ER -

Transforming growth factor-β (TGFβ) receptors I/II differentially regulate TGFβ1 and IGF-binding protein-3 mitogenic effects in the human placenta

Abstract

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