Projects per year
Abstract
The apparent mislocalization or excretion of cytoplasmic proteins is a commonly observed phenomenon in both bacteria and eukaryotes. However, reports on the mechanistic basis and the cellular function of this so called “non-classical protein secretion” are limited. Here we report that protein over-expression in recombinant cells and antibiotic-induced translation stress in wild-type E.
coli cells, both lead to excretion of cytoplasmic protein (ECP). Condition-specific metabolomic and proteomic analyses combined with genetic knockouts, indicate a role of both the large mechanosensitive channel (MscL) and the alternative ribosome-rescue factor A (ArfA) in ECP. Collectively, the findings indicate that the MscL-dependent protein excretion is positively regulated in response to both osmotic and arfA-mediated translational stress.
coli cells, both lead to excretion of cytoplasmic protein (ECP). Condition-specific metabolomic and proteomic analyses combined with genetic knockouts, indicate a role of both the large mechanosensitive channel (MscL) and the alternative ribosome-rescue factor A (ArfA) in ECP. Collectively, the findings indicate that the MscL-dependent protein excretion is positively regulated in response to both osmotic and arfA-mediated translational stress.
Original language | English |
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Article number | e02118-17 |
Journal | mBio |
Volume | 9 |
Issue number | 1 |
Early online date | 30 Jan 2018 |
DOIs | |
Publication status | Published - 2018 |
Research Beacons, Institutes and Platforms
- Manchester Institute of Biotechnology
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Dive into the research topics of 'Translation stress positively regulates MscL-dependent excretion of cytoplasmic proteins'. Together they form a unique fingerprint.Projects
- 2 Finished
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Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals
Scrutton, N., Azapagic, A., Balmer, A., Barran, P., Breitling, R., Delneri, D., Dixon, N., Faulon, J., Flitsch, S., Goble, C., Goodacre, R., Hay, S., Kell, D., Leys, D., Lloyd, J., Lockyer, N., Martin, P., Micklefield, J., Munro, A., Pedrosa Mendes, P., Randles, S., Salehi Yazdi, F., Shapira, P., Takano, E., Turner, N. & Winterburn, J.
14/11/14 → 13/05/20
Project: Research
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Development and Application of Next Generation Synthetic Biology Tools
1/11/13 → 31/10/19
Project: Research