Transport of dendrimer nanocarriers through epithelial cells via the transcellular route

Rachaneekorn Jevprasesphant; Jeffrey Penny; David Attwood; Antony D'Emanuele

doi:10.1016/j.jconrel.2004.03.022

Transport of dendrimer nanocarriers through epithelial cells via the transcellular route

Rachaneekorn Jevprasesphant, Jeffrey Penny, David Attwood, Antony D'Emanuele

Research output: Contribution to journal › Article › peer-review

Abstract

The mechanism of transport of G3 PAMAM and surface-modified (with lauroyl chains) G3 PAMAM dendrimer nanocarriers across Caco-2 cell monolayers has been investigated. Flow-cytometry studies following quenching of extracellular fluorescence demonstrated the cellular internalisation of dendrimers. Optical sectioning of cells incubated with fluorescein isothiocyanate (FITC)-conjugated dendrimer and lauroyl-dendrimer using confocal laser scanning microscopy revealed colocalisation of a marker for cell nuclei (4′,6-diamidino-2- phenylindole, DAPI) and FITC fluorescence, also suggesting cellular internalisation of dendrimers. Transmission electron microscopic analyses of cells incubated with gold-labelled G3 PAMAM dendrimers confirmed endocytosis-mediated cellular internalisation when dendrimers were applied to the apical domain of Caco-2 cells. These findings are in agreement with our previous studies using Caco-2 cell monolayers that showed a significant decrease of dendrimer uptake in the presence of colchicine (endocytosis inhibitor) and when temperature was reduced from 37 to 4°C. © 2004 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	259-267
Number of pages	8
Journal	Journal of Controlled Release
Volume	97
Issue number	2
DOIs	https://doi.org/10.1016/j.jconrel.2004.03.022
Publication status	Published - 18 Jun 2004

Keywords

Dendrimers
Drug delivery
Mechanism of transepithelial transport of dendrimer nanocarriers
Transcellular and paracellular routes

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10.1016/j.jconrel.2004.03.022

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@article{d85d893219474e33a5fcbedac5ecbf95,

title = "Transport of dendrimer nanocarriers through epithelial cells via the transcellular route",

abstract = "The mechanism of transport of G3 PAMAM and surface-modified (with lauroyl chains) G3 PAMAM dendrimer nanocarriers across Caco-2 cell monolayers has been investigated. Flow-cytometry studies following quenching of extracellular fluorescence demonstrated the cellular internalisation of dendrimers. Optical sectioning of cells incubated with fluorescein isothiocyanate (FITC)-conjugated dendrimer and lauroyl-dendrimer using confocal laser scanning microscopy revealed colocalisation of a marker for cell nuclei (4′,6-diamidino-2- phenylindole, DAPI) and FITC fluorescence, also suggesting cellular internalisation of dendrimers. Transmission electron microscopic analyses of cells incubated with gold-labelled G3 PAMAM dendrimers confirmed endocytosis-mediated cellular internalisation when dendrimers were applied to the apical domain of Caco-2 cells. These findings are in agreement with our previous studies using Caco-2 cell monolayers that showed a significant decrease of dendrimer uptake in the presence of colchicine (endocytosis inhibitor) and when temperature was reduced from 37 to 4°C. {\textcopyright} 2004 Elsevier B.V. All rights reserved.",

keywords = "Dendrimers, Drug delivery, Mechanism of transepithelial transport of dendrimer nanocarriers, Transcellular and paracellular routes",

author = "Rachaneekorn Jevprasesphant and Jeffrey Penny and David Attwood and Antony D'Emanuele",

year = "2004",

month = jun,

day = "18",

doi = "10.1016/j.jconrel.2004.03.022",

language = "English",

volume = "97",

pages = "259--267",

journal = "Journal of Controlled Release",

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TY - JOUR

T1 - Transport of dendrimer nanocarriers through epithelial cells via the transcellular route

AU - Jevprasesphant, Rachaneekorn

AU - Penny, Jeffrey

AU - Attwood, David

AU - D'Emanuele, Antony

PY - 2004/6/18

Y1 - 2004/6/18

N2 - The mechanism of transport of G3 PAMAM and surface-modified (with lauroyl chains) G3 PAMAM dendrimer nanocarriers across Caco-2 cell monolayers has been investigated. Flow-cytometry studies following quenching of extracellular fluorescence demonstrated the cellular internalisation of dendrimers. Optical sectioning of cells incubated with fluorescein isothiocyanate (FITC)-conjugated dendrimer and lauroyl-dendrimer using confocal laser scanning microscopy revealed colocalisation of a marker for cell nuclei (4′,6-diamidino-2- phenylindole, DAPI) and FITC fluorescence, also suggesting cellular internalisation of dendrimers. Transmission electron microscopic analyses of cells incubated with gold-labelled G3 PAMAM dendrimers confirmed endocytosis-mediated cellular internalisation when dendrimers were applied to the apical domain of Caco-2 cells. These findings are in agreement with our previous studies using Caco-2 cell monolayers that showed a significant decrease of dendrimer uptake in the presence of colchicine (endocytosis inhibitor) and when temperature was reduced from 37 to 4°C. © 2004 Elsevier B.V. All rights reserved.

AB - The mechanism of transport of G3 PAMAM and surface-modified (with lauroyl chains) G3 PAMAM dendrimer nanocarriers across Caco-2 cell monolayers has been investigated. Flow-cytometry studies following quenching of extracellular fluorescence demonstrated the cellular internalisation of dendrimers. Optical sectioning of cells incubated with fluorescein isothiocyanate (FITC)-conjugated dendrimer and lauroyl-dendrimer using confocal laser scanning microscopy revealed colocalisation of a marker for cell nuclei (4′,6-diamidino-2- phenylindole, DAPI) and FITC fluorescence, also suggesting cellular internalisation of dendrimers. Transmission electron microscopic analyses of cells incubated with gold-labelled G3 PAMAM dendrimers confirmed endocytosis-mediated cellular internalisation when dendrimers were applied to the apical domain of Caco-2 cells. These findings are in agreement with our previous studies using Caco-2 cell monolayers that showed a significant decrease of dendrimer uptake in the presence of colchicine (endocytosis inhibitor) and when temperature was reduced from 37 to 4°C. © 2004 Elsevier B.V. All rights reserved.

KW - Dendrimers

KW - Drug delivery

KW - Mechanism of transepithelial transport of dendrimer nanocarriers

KW - Transcellular and paracellular routes

U2 - 10.1016/j.jconrel.2004.03.022

DO - 10.1016/j.jconrel.2004.03.022

M3 - Article

SN - 0168-3659

VL - 97

SP - 259

EP - 267

JO - Journal of Controlled Release

JF - Journal of Controlled Release

IS - 2

ER -

Transport of dendrimer nanocarriers through epithelial cells via the transcellular route

Abstract

Keywords

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