Two distinct populations of tumor necrosis factor-stimulated gene-6 protein in the extracellular matrix of expanded mouse cumulus cell-oocyte complexes

D. Mukhopadhyay, V. C. Hascall, A. J. Day, A. Salustri, C. Fülöp

    Research output: Contribution to journalArticlepeer-review

    Abstract

    After the luteinizing hormone surge, the cumulus cell-oocyte complexes (COCs) in the preovulatory follicles produce a viscoelastic extracellular matrix, a process that requires the synthesis of hyaluronan as well as the incorporation of some components of the inter-α-trypsin inhibitor (IαI) family. In this study we report, that a hyaluronan-binding protein, the translated product of tumor necrosis factor-stimulated gene-6 (TSG-6), is also specifically accumulated in this matrix. TSG-6 mRNA expression is quickly upregulated and peaks at ∼1500 copies/cell 4 h after the ovulatory stimuli as assessed by quantitative reverse transcription-polymerase chain reaction. Immunohistochemistry reveals the colocalization of the TSG-6 protein and hyaluronan around the cumulus and granulosa cells. The TSG-6 protein exists in two distinct populations in the GaG matrix as demonstrated by Western-blot analysis. One population is a monomer that is anchored to the matrix by a noncovalent interaction. The second population is a covalent complex with either of the heavy chains of IαI and is bound to hyaluronan through a strong interaction that is resistant to denaturing conditions. The specific incorporation of the TSG-6 protein into the COG matrix suggests a structural role for this molecule. © 2001 Academic Press.
    Original languageEnglish
    Pages (from-to)173-181
    Number of pages8
    JournalArchives of Biochemistry and Biophysics
    Volume394
    Issue number2
    DOIs
    Publication statusPublished - 15 Oct 2001

    Keywords

    • Cumulus cell-oocyte complex
    • Extracellular matrix
    • Hyaluronan
    • Inter-α-trypsin inhibitor
    • Tumor necrosis factor-stimulated gene-6

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