Type VI collagen is present in human and bovine vitreous

David Mcleod; Paul Bishop; Shirley Ayad; Anthony Reardon; David McLeod; John Sheehan; Cay Kielty

doi:10.1007/BF00292358

Type VI collagen is present in human and bovine vitreous

David Mcleod, Paul Bishop, Shirley Ayad, Anthony Reardon, David McLeod, John Sheehan, Cay Kielty

Research output: Contribution to journal › Article › peer-review

Abstract

Background. Heterotypic (mixed) collagen fibrils that contain collagen types II, IX and V/XI have previously been identified in vitreous gel. The purpose of this study was to determine whether vitreous gel also contains type VI collagen microfibrils, as they are widely distributed in other tissues where they may play an important structural role. Methods. Bovine and human vitreous gel was analysed by rotary shadowing electron microscopy after bacterial collagenase digestion and gel filtration chromatography. Bovine vitreous was extracted in 4 M guanidine hydrochloride, and following isopycnic centrifugation fractions were analysed by western blotting. Results. Type VI collagen microfibrils were identified by electron microscopy in human and bovine vitreous. Type VI collagen was identified by western blot analysis. Conclusion. Type VI collagen is present in vitreous and may participate in the structural assembly of the vitreous gel.

Original language	English
Pages (from-to)	710-713
Number of pages	3
Journal	Graefe's Archive for Clinical and Experimental Ophthalmology
Volume	234
Issue number	11
DOIs	https://doi.org/10.1007/BF00292358
Publication status	Published - 1996

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title = "Type VI collagen is present in human and bovine vitreous",

abstract = "Background. Heterotypic (mixed) collagen fibrils that contain collagen types II, IX and V/XI have previously been identified in vitreous gel. The purpose of this study was to determine whether vitreous gel also contains type VI collagen microfibrils, as they are widely distributed in other tissues where they may play an important structural role. Methods. Bovine and human vitreous gel was analysed by rotary shadowing electron microscopy after bacterial collagenase digestion and gel filtration chromatography. Bovine vitreous was extracted in 4 M guanidine hydrochloride, and following isopycnic centrifugation fractions were analysed by western blotting. Results. Type VI collagen microfibrils were identified by electron microscopy in human and bovine vitreous. Type VI collagen was identified by western blot analysis. Conclusion. Type VI collagen is present in vitreous and may participate in the structural assembly of the vitreous gel.",

author = "David Mcleod and Paul Bishop and Shirley Ayad and Anthony Reardon and David McLeod and John Sheehan and Cay Kielty",

year = "1996",

doi = "10.1007/BF00292358",

language = "English",

volume = "234",

pages = "710--713",

journal = "Graefe's Archive for Clinical and Experimental Ophthalmology",

publisher = "Springer Nature",

number = "11",

}

TY - JOUR

T1 - Type VI collagen is present in human and bovine vitreous

AU - Mcleod, David

AU - Bishop, Paul

AU - Ayad, Shirley

AU - Reardon, Anthony

AU - McLeod, David

AU - Sheehan, John

AU - Kielty, Cay

PY - 1996

Y1 - 1996

N2 - Background. Heterotypic (mixed) collagen fibrils that contain collagen types II, IX and V/XI have previously been identified in vitreous gel. The purpose of this study was to determine whether vitreous gel also contains type VI collagen microfibrils, as they are widely distributed in other tissues where they may play an important structural role. Methods. Bovine and human vitreous gel was analysed by rotary shadowing electron microscopy after bacterial collagenase digestion and gel filtration chromatography. Bovine vitreous was extracted in 4 M guanidine hydrochloride, and following isopycnic centrifugation fractions were analysed by western blotting. Results. Type VI collagen microfibrils were identified by electron microscopy in human and bovine vitreous. Type VI collagen was identified by western blot analysis. Conclusion. Type VI collagen is present in vitreous and may participate in the structural assembly of the vitreous gel.

AB - Background. Heterotypic (mixed) collagen fibrils that contain collagen types II, IX and V/XI have previously been identified in vitreous gel. The purpose of this study was to determine whether vitreous gel also contains type VI collagen microfibrils, as they are widely distributed in other tissues where they may play an important structural role. Methods. Bovine and human vitreous gel was analysed by rotary shadowing electron microscopy after bacterial collagenase digestion and gel filtration chromatography. Bovine vitreous was extracted in 4 M guanidine hydrochloride, and following isopycnic centrifugation fractions were analysed by western blotting. Results. Type VI collagen microfibrils were identified by electron microscopy in human and bovine vitreous. Type VI collagen was identified by western blot analysis. Conclusion. Type VI collagen is present in vitreous and may participate in the structural assembly of the vitreous gel.

U2 - 10.1007/BF00292358

DO - 10.1007/BF00292358

M3 - Article

VL - 234

SP - 710

EP - 713

JO - Graefe's Archive for Clinical and Experimental Ophthalmology

JF - Graefe's Archive for Clinical and Experimental Ophthalmology

IS - 11

ER -

Type VI collagen is present in human and bovine vitreous

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