Unambiguous structural elucidation of base-modified purine nucleosides using NMR

Raman Narukulla; David E G Shuker; Vasudevan Ramesh; Yao Zhong Xu

doi:10.1002/mrc.2111

Unambiguous structural elucidation of base-modified purine nucleosides using NMR

Raman Narukulla, David E G Shuker, Vasudevan Ramesh, Yao Zhong Xu

Research output: Contribution to journal › Article › peer-review

Abstract

A general and unambiguous approach has been developed for structural elucidation of modified purine nucleosides using NMR spectroscopy. Systematic assignment of proton and carbon signals of modified nucleosides was firmly established by COSY and the anomerism of the glycosidic linkage of synthetic nucleosides clearly elucidated by NOESY experiments. Characteristic properties of 15N-isotopic labelling at specific positions of nucleosides were also employed for structural studies. The reported approach is applicable to other modified nucleosides and nucleotides, as well as nucleobases. Copyright © 2007 John Wiley & Sons, Ltd.

Original language	English
Pages (from-to)	1-8
Number of pages	7
Journal	Magnetic Resonance in Chemistry
Volume	46
Issue number	1
DOIs	https://doi.org/10.1002/mrc.2111
Publication status	Published - Jan 2008

Keywords

13C
15N
1H
Base
DNA
Modified purine
NMR
Nucleoside
Purine

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10.1002/mrc.2111

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AU - Xu, Yao Zhong

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AB - A general and unambiguous approach has been developed for structural elucidation of modified purine nucleosides using NMR spectroscopy. Systematic assignment of proton and carbon signals of modified nucleosides was firmly established by COSY and the anomerism of the glycosidic linkage of synthetic nucleosides clearly elucidated by NOESY experiments. Characteristic properties of 15N-isotopic labelling at specific positions of nucleosides were also employed for structural studies. The reported approach is applicable to other modified nucleosides and nucleotides, as well as nucleobases. Copyright © 2007 John Wiley & Sons, Ltd.

KW - 13C

KW - 15N

KW - 1H

KW - Base

KW - DNA

KW - Modified purine

KW - NMR

KW - Nucleoside

KW - Purine

U2 - 10.1002/mrc.2111

DO - 10.1002/mrc.2111

M3 - Article

SN - 1097-458X

VL - 46

SP - 1

EP - 8

JO - Magnetic Resonance in Chemistry

JF - Magnetic Resonance in Chemistry

IS - 1

ER -

Unambiguous structural elucidation of base-modified purine nucleosides using NMR

Abstract

Keywords

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