Understanding the Role of Yeast Yme1 in Mitochondrial Function Using Biochemical and Proteomics Analyses

Kwan Ting Kan, Michael Nelson, Christopher Grant, Simon Hubbard, Hui Lu (Corresponding)

Research output: Contribution to journalArticlepeer-review


Mitochondrial i-AAA proteinase Yme1 is a multifunctional protein that plays important roles in maintaining mitochondrial protein homeostasis and regulating biogenesis and function of mitochondrial proteins. However, due to the complex interplay of mitochondria and the multifunctional nature of Yme1, how Yme1 affects mitochondrial function and protein homeostasis is still poorly understood. In this study, we investigated how YME1 deletion affects yeast Saccharomyces cerevisiae growth, chronological life span, mitochondrial protein homeostasis and function, with a focus on the mitochondrial oxidative phosphorylation (OXPHOS) complexes. Our results show that whilst the YME1 deleted cells grow poorly under respiratory conditions, they grow similar to wild-type yeast under fermentative conditions. However, the chronological life span is impaired, indicating that Yme1 plays a key role in longevity. Using highly enriched mitochondrial extract and proteomic analysis, we show that the abundances of many mitochondrial proteins are altered by YME1 deletion. Several components of the respiratory chain complexes II, III, IV and V were significantly decreased, suggesting that Yme1 plays an important role in maintaining the level and function of complexes II-V. This result was confirmed using blue native-PAGE and in-solution-based enzyme activity assays. Taken together, this study shows that Yme1 plays an important role in the chronological life span and mitochondrial protein homeostasis and has deciphered its function in maintaining the activity of mitochondrial OXPHOS complexes.
Original languageEnglish
Article number13694
JournalInternational Journal of Molecular Sciences
Issue number22
Publication statusPublished - 8 Nov 2022


Dive into the research topics of 'Understanding the Role of Yeast Yme1 in Mitochondrial Function Using Biochemical and Proteomics Analyses'. Together they form a unique fingerprint.

Cite this