Unusual ECD fragmentation attributed to gas-phase helix formation in a conformationally dynamic peptide

Jason M D Kalapothakis; Yana Berezovskaya; Cleidiane G. Zampronio; Peter A. Faull; Perdita E. Barran; Helen J. Cooper

doi:10.1039/c3cc46356g

Unusual ECD fragmentation attributed to gas-phase helix formation in a conformationally dynamic peptide

Jason M D Kalapothakis, Yana Berezovskaya, Cleidiane G. Zampronio, Peter A. Faull, Perdita E. Barran, Helen J. Cooper

Research output: Contribution to journal › Article › peer-review

Abstract

The helix-forming character of a model decapeptide, L4PL 4K, is determined in the absence of solvent using ion mobility mass spectrometry, electron capture dissociation and molecular mechanics simulations. Unusual ECD fragmentation patterns dominated by b ions are attributed to helix formation upon electron capture and as a signature of conformational dynamics. © 2014 The Royal Society of Chemistry.

Original language	English
Pages (from-to)	198-200
Number of pages	2
Journal	Chemical Communications
Volume	50
Issue number	2
DOIs	https://doi.org/10.1039/c3cc46356g
Publication status	Published - 7 Jan 2014

Keywords

electron-capture dissociation
charged protein cations
tryptic peptides
in-vacuo
spectra
ions

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10.1039/c3cc46356g

http://www.ncbi.nlm.nih.gov/pubmed/24217599

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abstract = "The helix-forming character of a model decapeptide, L4PL 4K, is determined in the absence of solvent using ion mobility mass spectrometry, electron capture dissociation and molecular mechanics simulations. Unusual ECD fragmentation patterns dominated by b ions are attributed to helix formation upon electron capture and as a signature of conformational dynamics. {\textcopyright} 2014 The Royal Society of Chemistry.",

keywords = "electron-capture dissociation, charged protein cations, tryptic peptides, in-vacuo, spectra, ions",

author = "Kalapothakis, {Jason M D} and Yana Berezovskaya and Zampronio, {Cleidiane G.} and Faull, {Peter A.} and Barran, {Perdita E.} and Cooper, {Helen J.}",

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AU - Kalapothakis, Jason M D

AU - Berezovskaya, Yana

AU - Zampronio, Cleidiane G.

AU - Faull, Peter A.

AU - Barran, Perdita E.

AU - Cooper, Helen J.

N1 - 261LA Times Cited:0 Cited References Count:21

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N2 - The helix-forming character of a model decapeptide, L4PL 4K, is determined in the absence of solvent using ion mobility mass spectrometry, electron capture dissociation and molecular mechanics simulations. Unusual ECD fragmentation patterns dominated by b ions are attributed to helix formation upon electron capture and as a signature of conformational dynamics. © 2014 The Royal Society of Chemistry.

AB - The helix-forming character of a model decapeptide, L4PL 4K, is determined in the absence of solvent using ion mobility mass spectrometry, electron capture dissociation and molecular mechanics simulations. Unusual ECD fragmentation patterns dominated by b ions are attributed to helix formation upon electron capture and as a signature of conformational dynamics. © 2014 The Royal Society of Chemistry.

KW - electron-capture dissociation

KW - charged protein cations

KW - tryptic peptides

KW - in-vacuo

KW - spectra

KW - ions

U2 - 10.1039/c3cc46356g

DO - 10.1039/c3cc46356g

M3 - Article

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VL - 50

SP - 198

EP - 200

JO - Chemical Communications

JF - Chemical Communications

IS - 2

ER -

Unusual ECD fragmentation attributed to gas-phase helix formation in a conformationally dynamic peptide

Abstract

Keywords

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