Up-regulation of a H+-pyrophosphatase (H+-PPase) as a strategy to engineer drought-resistant crop plants

Sunghun Park; Jisheng Li; Jon K. Pittman; Gerald A. Berkowitz; Haibing Yang; Soledad Undurraga; Jay Morris; Kendal D. Hirschi; Roberto A. Gaxiola

doi:10.1073/pnas.0509512102

Up-regulation of a H+-pyrophosphatase (H+-PPase) as a strategy to engineer drought-resistant crop plants

Sunghun Park, Jisheng Li, Jon K. Pittman, Gerald A. Berkowitz, Haibing Yang, Soledad Undurraga, Jay Morris, Kendal D. Hirschi, Roberto A. Gaxiola

Research output: Contribution to journal › Article › peer-review

Abstract

Engineering drought-resistant crop plants is a critically important objective. Overexpression of the vacuolar H+-pyrophosphatase (H +-PPase) AVP1 in the model plant Arabidopsis thaliana results in enhanced performance under soil water deficits. Recent work demonstrates that AVP1 plays an important role in root development through the facilitation of auxin fluxes. With the objective of improving crop performance, we expressed AVP1 in a commercial cultivar of tomato. This approach resulted in (i) greater pyrophosphate-driven cation transport into root vacuolar fractions, (ii) increased root biomass, and (iii) enhanced recovery of plants from an episode of soil water deficit stress. More robust root systems allowed transgenic tomato plants to take up greater amounts of water during the imposed water deficit stress, resulting in a more favorable plant water status and less injury. This study documents a general strategy for improving drought resistance of crops. © 2005 by The National Academy of Sciences of the USA.

Original language	English
Pages (from-to)	18830-18835
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	102
Issue number	52
DOIs	https://doi.org/10.1073/pnas.0509512102
Publication status	Published - 27 Dec 2005

Keywords

Biotechnology
Root development
Tomato
Water deficit stress

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10.1073/pnas.0509512102

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Park, S., Li, J., Pittman, J. K., Berkowitz, G. A., Yang, H., Undurraga, S., Morris, J., Hirschi, K. D., & Gaxiola, R. A. (2005). Up-regulation of a H+-pyrophosphatase (H+-PPase) as a strategy to engineer drought-resistant crop plants. Proceedings of the National Academy of Sciences of the United States of America, 102(52), 18830-18835. https://doi.org/10.1073/pnas.0509512102

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title = "Up-regulation of a H+-pyrophosphatase (H+-PPase) as a strategy to engineer drought-resistant crop plants",

abstract = "Engineering drought-resistant crop plants is a critically important objective. Overexpression of the vacuolar H+-pyrophosphatase (H +-PPase) AVP1 in the model plant Arabidopsis thaliana results in enhanced performance under soil water deficits. Recent work demonstrates that AVP1 plays an important role in root development through the facilitation of auxin fluxes. With the objective of improving crop performance, we expressed AVP1 in a commercial cultivar of tomato. This approach resulted in (i) greater pyrophosphate-driven cation transport into root vacuolar fractions, (ii) increased root biomass, and (iii) enhanced recovery of plants from an episode of soil water deficit stress. More robust root systems allowed transgenic tomato plants to take up greater amounts of water during the imposed water deficit stress, resulting in a more favorable plant water status and less injury. This study documents a general strategy for improving drought resistance of crops. {\textcopyright} 2005 by The National Academy of Sciences of the USA.",

keywords = "Biotechnology, Root development, Tomato, Water deficit stress",

author = "Sunghun Park and Jisheng Li and Pittman, {Jon K.} and Berkowitz, {Gerald A.} and Haibing Yang and Soledad Undurraga and Jay Morris and Hirschi, {Kendal D.} and Gaxiola, {Roberto A.}",

year = "2005",

month = dec,

day = "27",

doi = "10.1073/pnas.0509512102",

language = "English",

volume = "102",

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journal = "Proceedings of the National Academy of Sciences of the United States of America",

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Park, S, Li, J, Pittman, JK, Berkowitz, GA, Yang, H, Undurraga, S, Morris, J, Hirschi, KD & Gaxiola, RA 2005, 'Up-regulation of a H+-pyrophosphatase (H+-PPase) as a strategy to engineer drought-resistant crop plants', Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 52, pp. 18830-18835. https://doi.org/10.1073/pnas.0509512102

TY - JOUR

T1 - Up-regulation of a H+-pyrophosphatase (H+-PPase) as a strategy to engineer drought-resistant crop plants

AU - Park, Sunghun

AU - Li, Jisheng

AU - Pittman, Jon K.

AU - Berkowitz, Gerald A.

AU - Yang, Haibing

AU - Undurraga, Soledad

AU - Morris, Jay

AU - Hirschi, Kendal D.

AU - Gaxiola, Roberto A.

PY - 2005/12/27

Y1 - 2005/12/27

N2 - Engineering drought-resistant crop plants is a critically important objective. Overexpression of the vacuolar H+-pyrophosphatase (H +-PPase) AVP1 in the model plant Arabidopsis thaliana results in enhanced performance under soil water deficits. Recent work demonstrates that AVP1 plays an important role in root development through the facilitation of auxin fluxes. With the objective of improving crop performance, we expressed AVP1 in a commercial cultivar of tomato. This approach resulted in (i) greater pyrophosphate-driven cation transport into root vacuolar fractions, (ii) increased root biomass, and (iii) enhanced recovery of plants from an episode of soil water deficit stress. More robust root systems allowed transgenic tomato plants to take up greater amounts of water during the imposed water deficit stress, resulting in a more favorable plant water status and less injury. This study documents a general strategy for improving drought resistance of crops. © 2005 by The National Academy of Sciences of the USA.

AB - Engineering drought-resistant crop plants is a critically important objective. Overexpression of the vacuolar H+-pyrophosphatase (H +-PPase) AVP1 in the model plant Arabidopsis thaliana results in enhanced performance under soil water deficits. Recent work demonstrates that AVP1 plays an important role in root development through the facilitation of auxin fluxes. With the objective of improving crop performance, we expressed AVP1 in a commercial cultivar of tomato. This approach resulted in (i) greater pyrophosphate-driven cation transport into root vacuolar fractions, (ii) increased root biomass, and (iii) enhanced recovery of plants from an episode of soil water deficit stress. More robust root systems allowed transgenic tomato plants to take up greater amounts of water during the imposed water deficit stress, resulting in a more favorable plant water status and less injury. This study documents a general strategy for improving drought resistance of crops. © 2005 by The National Academy of Sciences of the USA.

KW - Biotechnology

KW - Root development

KW - Tomato

KW - Water deficit stress

U2 - 10.1073/pnas.0509512102

DO - 10.1073/pnas.0509512102

M3 - Article

C2 - 16361442

SN - 0027-8424

VL - 102

SP - 18830

EP - 18835

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 52

ER -

Up-regulation of a H+-pyrophosphatase (H+-PPase) as a strategy to engineer drought-resistant crop plants

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Keywords

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