Use of expressed sequence tag analysis and cDNA microarrays of the filamentous fungus Aspergillus nidulans

Andrew H. Sims, Geoffrey D. Robson, David C. Hoyle, Stephen G. Oliver, Geoffrey Turner, Rolf A. Prade, Hugh H. Russell, Nigel S. Dunn-Coleman, Manda E. Gent

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The use of microarrays in the analysis of gene expression is becoming widespread for many organisms, including yeast. However, although the genomes of a number of filamentous fungi have been fully or partially sequenced, microarray analysis is still in its infancy in these organisms. Here, we describe the construction and validation of microarrays for the fungus Aspergillus nidulans using PCR products from a 4092 EST conidial germination library. An experiment was designed to validate these arrays by monitoring the expression profiles of known genes following the addition of 1% (w/v) glucose to wild-type A. nidulans cultures grown to mid-exponential phase in Vogel's minimal medium with ethanol as the sole carbon source. The profiles of genes showing statistically significant differential expression following the glucose up-shift are presented and an assessment of the quality and reproducibility of the A. nidulans arrays discussed. © 2003 Elsevier Inc. All rights reserved.
    Original languageEnglish
    Pages (from-to)199-212
    Number of pages13
    JournalFungal Genetics and Biology
    Volume41
    Issue number2
    DOIs
    Publication statusPublished - Feb 2004

    Keywords

    • Alcohol
    • Aspergillus nidulans
    • creA
    • EST
    • Ethanol
    • Glucose repression
    • Microarray

    Research Beacons, Institutes and Platforms

    • Manchester Institute of Biotechnology

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