Use of the TLX ultracentrifuge for the isolation of different density lipoproteins and effects of freeze/thawing of human plasma before ultracentrifugation

Valentine Charlton-Menys, Jelena Chobotova, Paul N. Durrington

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Background: Isolation of different density lipoproteins by ultracentrifugation can require lengthy centrifugation times and freeze/thawing of plasma may influence recovery. Methods: We isolated a range of lipoproteins using a preparative ultracentrifuge and the TLX micro-ultracentrifuge and determined the effect of freeze/thawing of plasma beforehand. Results: In fresh plasma, there was no significant difference in results for small-dense low-density lipoprotein apolipoprotein B (LDL apoB) (density >1.044 g/mL) or cholesterol at density >1.006 g/mL. Freeze/thawing had no effect on closely correlated results for small-dense LDL apoB (r=0.85; p
    Original languageEnglish
    Pages (from-to)1285-1288
    Number of pages3
    JournalClinical Chemistry and Laboratory Medicine
    Volume46
    Issue number9
    DOIs
    Publication statusPublished - 1 Sept 2008

    Keywords

    • High-density lipoprotein (HDL)
    • Lipoproteins
    • Non-HDL cholesterol
    • Small-dense low-density lipoprotein
    • Ultracentrifugation
    • Very low-density lipoprotein

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