Using FTIR spectroscopy for rapid determination of lipid accumulation in response to nitrogen limitation in freshwater microalgae

Andrew P. Dean; David C. Sigee; Beatriz Estrada; Jon K. Pittman

doi:10.1016/j.biortech.2010.01.065

Using FTIR spectroscopy for rapid determination of lipid accumulation in response to nitrogen limitation in freshwater microalgae

Andrew P. Dean, David C. Sigee, Beatriz Estrada, Jon K. Pittman

Research output: Contribution to journal › Article › peer-review

Abstract

In this study Fourier transform infrared micro-spectroscopy (FTIR) was used to determine lipid and carbohydrate content over time in the freshwater microalgae Chlamydomonas reinhardtii and Scenedesmus subspicatus grown in batch culture in limiting concentrations of nitrogen (N). Both algae exhibited restricted cell division and increased cell size following N-limitation. FTIR spectra of cells in N-limited media showed increasing lipid:amide I and carbohydrate:amide I ratios over time. The use of lipid- and starch-staining dyes confirmed that the observed ratio changes were due to increased lipid and carbohydrate synthesis. These results demonstrate rapid metabolic responses of C. reinhardtii and S. subspicatus to changing nutrient availability, and indicate the efficiency of FTIR as a reliable method for high-throughput determination of lipid induction. © 2010 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	4499-4507
Number of pages	8
Journal	Bioresource Technology
Volume	101
Issue number	12
DOIs	https://doi.org/10.1016/j.biortech.2010.01.065
Publication status	Published - Jun 2010

Keywords

Biofuel
Fourier transform infrared spectroscopy
Lipid biosynthesis
Microalgae
Nitrogen limitation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2010.01.065

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title = "Using FTIR spectroscopy for rapid determination of lipid accumulation in response to nitrogen limitation in freshwater microalgae",

abstract = "In this study Fourier transform infrared micro-spectroscopy (FTIR) was used to determine lipid and carbohydrate content over time in the freshwater microalgae Chlamydomonas reinhardtii and Scenedesmus subspicatus grown in batch culture in limiting concentrations of nitrogen (N). Both algae exhibited restricted cell division and increased cell size following N-limitation. FTIR spectra of cells in N-limited media showed increasing lipid:amide I and carbohydrate:amide I ratios over time. The use of lipid- and starch-staining dyes confirmed that the observed ratio changes were due to increased lipid and carbohydrate synthesis. These results demonstrate rapid metabolic responses of C. reinhardtii and S. subspicatus to changing nutrient availability, and indicate the efficiency of FTIR as a reliable method for high-throughput determination of lipid induction. {\textcopyright} 2010 Elsevier Ltd. All rights reserved.",

keywords = "Biofuel, Fourier transform infrared spectroscopy, Lipid biosynthesis, Microalgae, Nitrogen limitation",

author = "Dean, {Andrew P.} and Sigee, {David C.} and Beatriz Estrada and Pittman, {Jon K.}",

year = "2010",

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doi = "10.1016/j.biortech.2010.01.065",

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volume = "101",

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journal = "Bioresource Technology",

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TY - JOUR

T1 - Using FTIR spectroscopy for rapid determination of lipid accumulation in response to nitrogen limitation in freshwater microalgae

AU - Dean, Andrew P.

AU - Sigee, David C.

AU - Estrada, Beatriz

AU - Pittman, Jon K.

PY - 2010/6

Y1 - 2010/6

N2 - In this study Fourier transform infrared micro-spectroscopy (FTIR) was used to determine lipid and carbohydrate content over time in the freshwater microalgae Chlamydomonas reinhardtii and Scenedesmus subspicatus grown in batch culture in limiting concentrations of nitrogen (N). Both algae exhibited restricted cell division and increased cell size following N-limitation. FTIR spectra of cells in N-limited media showed increasing lipid:amide I and carbohydrate:amide I ratios over time. The use of lipid- and starch-staining dyes confirmed that the observed ratio changes were due to increased lipid and carbohydrate synthesis. These results demonstrate rapid metabolic responses of C. reinhardtii and S. subspicatus to changing nutrient availability, and indicate the efficiency of FTIR as a reliable method for high-throughput determination of lipid induction. © 2010 Elsevier Ltd. All rights reserved.

AB - In this study Fourier transform infrared micro-spectroscopy (FTIR) was used to determine lipid and carbohydrate content over time in the freshwater microalgae Chlamydomonas reinhardtii and Scenedesmus subspicatus grown in batch culture in limiting concentrations of nitrogen (N). Both algae exhibited restricted cell division and increased cell size following N-limitation. FTIR spectra of cells in N-limited media showed increasing lipid:amide I and carbohydrate:amide I ratios over time. The use of lipid- and starch-staining dyes confirmed that the observed ratio changes were due to increased lipid and carbohydrate synthesis. These results demonstrate rapid metabolic responses of C. reinhardtii and S. subspicatus to changing nutrient availability, and indicate the efficiency of FTIR as a reliable method for high-throughput determination of lipid induction. © 2010 Elsevier Ltd. All rights reserved.

KW - Biofuel

KW - Fourier transform infrared spectroscopy

KW - Lipid biosynthesis

KW - Microalgae

KW - Nitrogen limitation

U2 - 10.1016/j.biortech.2010.01.065

DO - 10.1016/j.biortech.2010.01.065

M3 - Article

C2 - 20153176

SN - 0960-8524

VL - 101

SP - 4499

EP - 4507

JO - Bioresource Technology

JF - Bioresource Technology

IS - 12

ER -

Using FTIR spectroscopy for rapid determination of lipid accumulation in response to nitrogen limitation in freshwater microalgae

Abstract

Keywords

UN SDGs

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