UT-B is expressed in bovine rumen: Potential role in ruminal urea transport

G. S. Stewart; C. Graham; S. Cattell; T. P L Smith; N. L. Simmons; C. P. Smith

doi:10.1152/ajpregu.00127.2005

UT-B is expressed in bovine rumen: Potential role in ruminal urea transport

G. S. Stewart, C. Graham, S. Cattell, T. P L Smith, N. L. Simmons, C. P. Smith

Research output: Contribution to journal › Article › peer-review

Abstract

The UT-A (SLC14a2) and UT-B (SLC14a1) genes encode a family of specialized urea transporter proteins that regulate urea movement across plasma membranes. In this report, we describe the structure of the bovine UT-B (bUT-B) gene and characterize UT-B expression in bovine rumen. Northern analysis using a full-length bUT-B probe detected a 3.7-kb UT-B signal in rumen. RT-PCR of bovine mRNA revealed the presence of two UT-B splice variants, bUT-B1 and bUT-B2, with bUT-B2 the predominant variant in rumen. Immunoblotting studies of bovine rumen tissue, using an antibody targeted to the NH2-terminus of mouse UT-B, confirmed the presence of 43- to 54-kDa UT-B proteins. Immunolocalization studies showed that UT-B was mainly located on cell plasma membranes in epithelial layers of the bovine rumen. Using chamber measurements of ruminal transepithelial transport of 14C-labeled urea indicated that urea flux was characteristically inhibited by phloretin. We conclude that bUT-B is expressed in the bovine rumen and may function to transport urea into the rumen as part of the ruminant urea nitrogen salvaging process.

Original language	English
Pages (from-to)	R605-R612
Journal	AJP: Regulatory, Integrative and Comparative Physiology
Volume	289
Issue number	2
DOIs	https://doi.org/10.1152/ajpregu.00127.2005
Publication status	Published - Aug 2005

Keywords

Facilitative transporter
Nitrogen balance
Phloretin
Thionicotinamide
Transepithelial flux

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10.1152/ajpregu.00127.2005

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title = "UT-B is expressed in bovine rumen: Potential role in ruminal urea transport",

abstract = "The UT-A (SLC14a2) and UT-B (SLC14a1) genes encode a family of specialized urea transporter proteins that regulate urea movement across plasma membranes. In this report, we describe the structure of the bovine UT-B (bUT-B) gene and characterize UT-B expression in bovine rumen. Northern analysis using a full-length bUT-B probe detected a 3.7-kb UT-B signal in rumen. RT-PCR of bovine mRNA revealed the presence of two UT-B splice variants, bUT-B1 and bUT-B2, with bUT-B2 the predominant variant in rumen. Immunoblotting studies of bovine rumen tissue, using an antibody targeted to the NH2-terminus of mouse UT-B, confirmed the presence of 43- to 54-kDa UT-B proteins. Immunolocalization studies showed that UT-B was mainly located on cell plasma membranes in epithelial layers of the bovine rumen. Using chamber measurements of ruminal transepithelial transport of 14C-labeled urea indicated that urea flux was characteristically inhibited by phloretin. We conclude that bUT-B is expressed in the bovine rumen and may function to transport urea into the rumen as part of the ruminant urea nitrogen salvaging process.",

keywords = "Facilitative transporter, Nitrogen balance, Phloretin, Thionicotinamide, Transepithelial flux",

author = "Stewart, {G. S.} and C. Graham and S. Cattell and Smith, {T. P L} and Simmons, {N. L.} and Smith, {C. P.}",

year = "2005",

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language = "English",

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AU - Stewart, G. S.

AU - Graham, C.

AU - Cattell, S.

AU - Smith, T. P L

AU - Simmons, N. L.

AU - Smith, C. P.

PY - 2005/8

Y1 - 2005/8

N2 - The UT-A (SLC14a2) and UT-B (SLC14a1) genes encode a family of specialized urea transporter proteins that regulate urea movement across plasma membranes. In this report, we describe the structure of the bovine UT-B (bUT-B) gene and characterize UT-B expression in bovine rumen. Northern analysis using a full-length bUT-B probe detected a 3.7-kb UT-B signal in rumen. RT-PCR of bovine mRNA revealed the presence of two UT-B splice variants, bUT-B1 and bUT-B2, with bUT-B2 the predominant variant in rumen. Immunoblotting studies of bovine rumen tissue, using an antibody targeted to the NH2-terminus of mouse UT-B, confirmed the presence of 43- to 54-kDa UT-B proteins. Immunolocalization studies showed that UT-B was mainly located on cell plasma membranes in epithelial layers of the bovine rumen. Using chamber measurements of ruminal transepithelial transport of 14C-labeled urea indicated that urea flux was characteristically inhibited by phloretin. We conclude that bUT-B is expressed in the bovine rumen and may function to transport urea into the rumen as part of the ruminant urea nitrogen salvaging process.

AB - The UT-A (SLC14a2) and UT-B (SLC14a1) genes encode a family of specialized urea transporter proteins that regulate urea movement across plasma membranes. In this report, we describe the structure of the bovine UT-B (bUT-B) gene and characterize UT-B expression in bovine rumen. Northern analysis using a full-length bUT-B probe detected a 3.7-kb UT-B signal in rumen. RT-PCR of bovine mRNA revealed the presence of two UT-B splice variants, bUT-B1 and bUT-B2, with bUT-B2 the predominant variant in rumen. Immunoblotting studies of bovine rumen tissue, using an antibody targeted to the NH2-terminus of mouse UT-B, confirmed the presence of 43- to 54-kDa UT-B proteins. Immunolocalization studies showed that UT-B was mainly located on cell plasma membranes in epithelial layers of the bovine rumen. Using chamber measurements of ruminal transepithelial transport of 14C-labeled urea indicated that urea flux was characteristically inhibited by phloretin. We conclude that bUT-B is expressed in the bovine rumen and may function to transport urea into the rumen as part of the ruminant urea nitrogen salvaging process.

KW - Facilitative transporter

KW - Nitrogen balance

KW - Phloretin

KW - Thionicotinamide

KW - Transepithelial flux

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DO - 10.1152/ajpregu.00127.2005

M3 - Article

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SP - R605-R612

JO - AJP: Regulatory, Integrative and Comparative Physiology

JF - AJP: Regulatory, Integrative and Comparative Physiology

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ER -

UT-B is expressed in bovine rumen: Potential role in ruminal urea transport

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Keywords

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