Vascular endothelial growth factor production is regulated by gene polymorphisms

Mojgan Mohammadi; Mohammad Reza Bazrafshani; Philip J. Day; William E R Ollier

Vascular endothelial growth factor production is regulated by gene polymorphisms

Mojgan Mohammadi, Mohammad Reza Bazrafshani, Philip J. Day, William E R Ollier

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Vascular endothelial growth factor (VEGF) has a key role in angiogene-sis and in transplantation. The level of VEGF is related to the differences in the DNA sequence of its promoter region. Objectives: In this study, the association between the combination of VEGF -1154 G and -2578 C alleles and VEGF production by LPS-stimulated PBMCs was investigated. In addition; the relationship between VEGF poly-morphisms and the influence of TNF-α and IL-4 on VEGF production was studied. Methods: VEGF -1154 G/A and -2578 C/A were detected using ARMS-PCR. To de-termine the impact of combinations of these two polymorphisms on VEGF production; PBMCs were stimulated by LPS and VEGF production was measured by ELISA. Re-sults: The combinations of -1154 GG/-2578 CC and -1154 GG/-2578 CA were signifi-cantly associated with higher VEGF production (p

Original language	English
Pages (from-to)	119-129
Number of pages	10
Journal	Iranian Journal of Immunology
Volume	6
Issue number	3
Publication status	Published - Sept 2009

Keywords

IL-4
TNF-α
VEGF

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title = "Vascular endothelial growth factor production is regulated by gene polymorphisms",

abstract = "Background: Vascular endothelial growth factor (VEGF) has a key role in angiogene-sis and in transplantation. The level of VEGF is related to the differences in the DNA sequence of its promoter region. Objectives: In this study, the association between the combination of VEGF -1154 G and -2578 C alleles and VEGF production by LPS-stimulated PBMCs was investigated. In addition; the relationship between VEGF poly-morphisms and the influence of TNF-α and IL-4 on VEGF production was studied. Methods: VEGF -1154 G/A and -2578 C/A were detected using ARMS-PCR. To de-termine the impact of combinations of these two polymorphisms on VEGF production; PBMCs were stimulated by LPS and VEGF production was measured by ELISA. Re-sults: The combinations of -1154 GG/-2578 CC and -1154 GG/-2578 CA were signifi-cantly associated with higher VEGF production (p",

keywords = "IL-4, TNF-α, VEGF",

author = "Mojgan Mohammadi and Bazrafshani, {Mohammad Reza} and Day, {Philip J.} and Ollier, {William E R}",

year = "2009",

month = sep,

language = "English",

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pages = "119--129",

journal = "Iranian Journal of Immunology",

issn = "1735-1383",

publisher = "Shiraz University of Medical Sciences",

number = "3",

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TY - JOUR

T1 - Vascular endothelial growth factor production is regulated by gene polymorphisms

AU - Mohammadi, Mojgan

AU - Bazrafshani, Mohammad Reza

AU - Day, Philip J.

AU - Ollier, William E R

PY - 2009/9

Y1 - 2009/9

N2 - Background: Vascular endothelial growth factor (VEGF) has a key role in angiogene-sis and in transplantation. The level of VEGF is related to the differences in the DNA sequence of its promoter region. Objectives: In this study, the association between the combination of VEGF -1154 G and -2578 C alleles and VEGF production by LPS-stimulated PBMCs was investigated. In addition; the relationship between VEGF poly-morphisms and the influence of TNF-α and IL-4 on VEGF production was studied. Methods: VEGF -1154 G/A and -2578 C/A were detected using ARMS-PCR. To de-termine the impact of combinations of these two polymorphisms on VEGF production; PBMCs were stimulated by LPS and VEGF production was measured by ELISA. Re-sults: The combinations of -1154 GG/-2578 CC and -1154 GG/-2578 CA were signifi-cantly associated with higher VEGF production (p

AB - Background: Vascular endothelial growth factor (VEGF) has a key role in angiogene-sis and in transplantation. The level of VEGF is related to the differences in the DNA sequence of its promoter region. Objectives: In this study, the association between the combination of VEGF -1154 G and -2578 C alleles and VEGF production by LPS-stimulated PBMCs was investigated. In addition; the relationship between VEGF poly-morphisms and the influence of TNF-α and IL-4 on VEGF production was studied. Methods: VEGF -1154 G/A and -2578 C/A were detected using ARMS-PCR. To de-termine the impact of combinations of these two polymorphisms on VEGF production; PBMCs were stimulated by LPS and VEGF production was measured by ELISA. Re-sults: The combinations of -1154 GG/-2578 CC and -1154 GG/-2578 CA were signifi-cantly associated with higher VEGF production (p

KW - IL-4

KW - TNF-α

KW - VEGF

M3 - Article

C2 - 19801785

SN - 1735-1383

VL - 6

SP - 119

EP - 129

JO - Iranian Journal of Immunology

JF - Iranian Journal of Immunology

IS - 3

ER -

Vascular endothelial growth factor production is regulated by gene polymorphisms

Abstract

Keywords

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