Vascular endothelial growth factor production is regulated by gene polymorphisms

Mojgan Mohammadi, Mohammad Reza Bazrafshani, Philip J. Day, William E R Ollier

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Background: Vascular endothelial growth factor (VEGF) has a key role in angiogene-sis and in transplantation. The level of VEGF is related to the differences in the DNA sequence of its promoter region. Objectives: In this study, the association between the combination of VEGF -1154 G and -2578 C alleles and VEGF production by LPS-stimulated PBMCs was investigated. In addition; the relationship between VEGF poly-morphisms and the influence of TNF-α and IL-4 on VEGF production was studied. Methods: VEGF -1154 G/A and -2578 C/A were detected using ARMS-PCR. To de-termine the impact of combinations of these two polymorphisms on VEGF production; PBMCs were stimulated by LPS and VEGF production was measured by ELISA. Re-sults: The combinations of -1154 GG/-2578 CC and -1154 GG/-2578 CA were signifi-cantly associated with higher VEGF production (p
    Original languageEnglish
    Pages (from-to)119-129
    Number of pages10
    JournalIranian Journal of Immunology
    Volume6
    Issue number3
    Publication statusPublished - Sep 2009

    Keywords

    • IL-4
    • TNF-α
    • VEGF

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