@article{ecba17709f1046eeb6e39b31a77af491,
title = "Vesicle aggregation by multivalent ligands: Relating crosslinking ability to surface affinity",
abstract = "In an effort to improve the stability of our tissue-mimetic vesicle aggregates, we have investigated how increasing the valency of our multivalent crosslinking ligand, poly-l-histidine, affected both the extent of vesicle aggregation and the affinity of the multivalent ligand for the synthetic receptor Cu(1) embedded in the vesicle membranes. Although increasing ligand valency gave the anticipated increase in the size of the vesicle aggregates, isothermal calorimetric studies did not show the expected increase in the valence-corrected binding constant for the embedded receptors. To explain both observations, we have developed a simple new binding model that encompasses both multivalent binding to receptors on a single vesicle surface (intramembrane binding) and vesicle crosslinking (intermembrane binding). This journal is {\textcopyright} The Royal Society of Chemistry.",
keywords = "Animal tissue (-mimetic, tissue-mimetic vesicle aggregation by multivalent ligands and relating crosslinking ability to surface affinity), Affinity, Aggregation, Association enthalpy, Association entropy, Calorimetry, Formation constant, Liposomes, Simulation and Modeling, Stability (tissue-mimetic vesicle aggregation by multivalent ligands and relating crosslinking ability to surface affinity), Ligands Role: BUU (Biological use, unclassified), PEP (Physical, engineering or chemical process), PRP (Properties), BIOL (Biological study), PROC (Process), USES (Uses) (tissue-mimetic vesicle aggregation by multivalent ligands and relating crosslinkin, vesicle aggregation multivalent ligand crosslinking surface affinity",
author = "Xi Wang and Mart, {Robert J.} and Webb, {Simon J.}",
note = "CAN 147:359841 9-16 Biochemical Methods Journal 1477-0520 written in English 26062-48-6D (Poly-L-histidine); 26854-81-9D Role: BUU (Biological use, unclassified), PEP (Physical, engineering or chemical process), PRP (Properties), BIOL (Biological study), PROC (Process), USES (Uses) (crosslinking ligand; tissue-mimetic vesicle aggregation by multivalent ligands and relating crosslinking ability to surface affinity); 2497-02-1; 949491-92-3 Role: BUU (Biological use, unclassified), PEP (Physical, engineering or chemical process), PRP (Properties), BIOL (Biological study), PROC (Process), USES (Uses) (ligand; tissue-mimetic vesicle aggregation by multivalent ligands and relating crosslinking ability to surface affinity); 917379-09-0P Role: BUU (Biological use, unclassified), PEP (Physical, engineering or chemical process), PRP (Properties), SPN (Synthetic preparation), BIOL (Biological study), PREP (Preparation), PROC (Process), USES (Uses) (receptor; tissue-mimetic vesicle aggregation by multivalent ligands and relating crosslinking ability to surface affinity); 76-05-1 (Trifluoroacetic acid); 112-60-7 (Tetra(ethylene glycol)); 3443-45-6 (1-Pyrenebutyric acid); 85916-13-8 Role: RCT (Reactant), RACT (Reactant or reagent) (tissue-mimetic vesicle aggregation by multivalent ligands and relating crosslinking ability to surface affinity); 77544-60-6P; 870150-75-7P; 949110-86-5P; 949110-87-6P; 949110-88-7P Role: RCT (Reactant), SPN (Synthetic preparation), PREP (Preparation), RACT (Reactant or reagent) (tissue-mimetic vesicle aggregation by multivalent ligands and relating crosslinking ability to surface affinity)",
year = "2007",
doi = "10.1039/b706662g",
language = "English",
volume = "5",
pages = "2498--2505",
journal = "Organic and Biomolecular Chemistry",
issn = "1477-0520",
publisher = "Royal Society of Chemistry",
number = "15",
}