Vinculin is required to maintain glomerular barrier integrity

Franziska Lausecker, Xuefei Tian, Kazunori Inoue, Zhen Wang, Christopher E. Pedigo, Hossam Hassan, Chang Liu, Margaret Zimmer, Stephanie Jinno, Abby L Huckle, Hellyeh Hamidi, Robert S. Ross, Roy Zent, Christoph Ballestrem, Rachel Lennon, Shuta Ishibe

Research output: Contribution to journalArticlepeer-review


Cell-matrix interactions and podocyte intercellular junctions are key for maintaining the glomerular filtration barrier. Vinculin, a cytoplasmic protein, couples actin filaments to integrin-mediated cell-matrix adhesions and to cadherin-based intercellular junctions. Here, we examined the role of vinculin in podocytes by the generation of a podocyte-specific knockout mouse. Mice lacking podocyte vinculin had increased albuminuria and foot process effacement following injury in vivo. Analysis of primary podocytes isolated from the mutant mice revealed defects in cell protrusions, altered focal adhesion size and signaling, as well as impaired cell migration. Furthermore, we found a marked mislocalization of the intercellular junction protein zonula occludens-1. In kidney sections from patients with focal segmental glomerulosclerosis, minimal change disease and membranous nephropathy, we observed dramatic differences in the expression levels and localization of vinculin. Thus, our results suggest that vinculin is necessary to maintain the integrity of the glomerular filtration barrier by modulating podocyte foot processes and stabilizing intercellular junctions.
Original languageEnglish
JournalKidney International
Early online date12 Dec 2017
Publication statusPublished - 2017


  • glomerular disease
  • podocyte
  • cell adhesion
  • vinculin
  • glomerular filtration barrier


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