Virus free, cell-based assay for the quantification of murine type I interferons

Mariela Bollati-Fogolín, Werner Müller

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Interferons (IFNs) are potent biologically active proteins synthesized and secreted by somatic cells of all mammalian species. They have been well characterized, with respect to structure, biological activities and clinical therapeutic effects. Historically, IFN antiviral activity assays were the first type of bioassays developed to measure the relative activity or potency of IFN preparations. Here we report a new virus free, cell-based assay to quantify murine type I IFN. It basically consists of an indicator cell line in which the Cre-recombinase is driven by the IFN-inducible Mx1 promoter and, when activated, deletes a stop cassette upstream of the eGFP coding region, resulting in the expression of eGFP. The percentage of eGFP expressing cells accurately correlates to the amount of type I IFN added to the culture and can easily be monitored. © 2005 Elsevier B.V. All rights reserved.
    Original languageEnglish
    Pages (from-to)169-175
    Number of pages6
    JournalJournal of immunological methods
    Volume306
    Issue number1-2
    DOIs
    Publication statusPublished - 30 Nov 2005

    Keywords

    • Cre-recombinase
    • eGFP
    • Immortalized fibroblast cells
    • Murine type I IFN
    • Reporter gene assay

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