Wavefront‐sensorless adaptive optics with a laser‐free spinning disk confocal microscope

Syed Asad Hussain, Toshiki Kubo, Nicholas Hall, Dalia Gala, Karen Mary Hampson, Richard Parton, Mick A. Phillips, Matthew Wincott, Katsumasa Fujita, Ilan Davis, Ian Dobbie, Martin J. Booth

Research output: Contribution to journalArticlepeer-review

Abstract

Adaptive optics is being applied widely to a range of microscopies in order to improve imaging quality in the presence of specimen-induced aberrations. We present here the first implementation of wavefront-sensorless adaptive optics for a laser-free, aperture correlation, spinning disk microscope. This widefield method provides confocal-like optical sectioning through use of a patterned disk in the illumination and detection paths. Like other high-resolution microscopes, its operation is compromised by aberrations due to refractive index mismatch and variations within the specimen. Correction of such aberrations shows improved signal level, contrast and resolution.
Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalJournal of Microscopy
Early online date30 Oct 2020
DOIs
Publication statusPublished - 20 Nov 2020

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