Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s

Martin J. Weissenborn; Sandra Notonier; Sarah Luise Lang; Konrad B. Otte; Susanne Herter; Nicholas Turner; Sabine Flitsch; Bernhard Hauer

doi:10.1039/c6cc01749e

Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s

Martin J. Weissenborn, Sandra Notonier, Sarah Luise Lang, Konrad B. Otte, Susanne Herter, Nicholas Turner, Sabine Flitsch, Bernhard Hauer^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

A readily available galactose oxidase (GOase) variant was used to develop a whole cell screening assay. This endpoint detection system was applied in a proof-of-concept approach by screening a focussed mutant library. This led to the discovery of the thus far most active P450 Marinobacter aquaeolei mutant catalysing the terminal hydroxylation of fatty acids.

Original language	English
Pages (from-to)	6158-6161
Number of pages	4
Journal	Chemical Communications
Volume	52
Issue number	36
Early online date	7 Apr 2016
DOIs	https://doi.org/10.1039/c6cc01749e
Publication status	Published - 2016

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abstract = "A readily available galactose oxidase (GOase) variant was used to develop a whole cell screening assay. This endpoint detection system was applied in a proof-of-concept approach by screening a focussed mutant library. This led to the discovery of the thus far most active P450 Marinobacter aquaeolei mutant catalysing the terminal hydroxylation of fatty acids.",

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AU - Weissenborn, Martin J.

AU - Notonier, Sandra

AU - Lang, Sarah Luise

AU - Otte, Konrad B.

AU - Herter, Susanne

AU - Turner, Nicholas

AU - Flitsch, Sabine

AU - Hauer, Bernhard

PY - 2016

Y1 - 2016

N2 - A readily available galactose oxidase (GOase) variant was used to develop a whole cell screening assay. This endpoint detection system was applied in a proof-of-concept approach by screening a focussed mutant library. This led to the discovery of the thus far most active P450 Marinobacter aquaeolei mutant catalysing the terminal hydroxylation of fatty acids.

AB - A readily available galactose oxidase (GOase) variant was used to develop a whole cell screening assay. This endpoint detection system was applied in a proof-of-concept approach by screening a focussed mutant library. This led to the discovery of the thus far most active P450 Marinobacter aquaeolei mutant catalysing the terminal hydroxylation of fatty acids.

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U2 - 10.1039/c6cc01749e

DO - 10.1039/c6cc01749e

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VL - 52

SP - 6158

EP - 6161

JO - Chemical Communications

JF - Chemical Communications

IS - 36

ER -

Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s

Abstract

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