X-ray phase microtomography with a single grating for high-throughput investigations of biological tissue

Marie-christine Zdora, Joan Vila-comamala, Georg Schulz, Anna Khimchenko, Alexander Hipp, Andrew C. Cook, Daniel Dilg, Christian David, Christian Grünzweig, Christoph Rau, Pierre Thibault, Irene Zanette

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The high-throughput 3D visualisation of biological specimens is essential for studying diseases and developmental disorders. It requires imaging methods that deliver high-contrast, high-resolution volumetric information at short sample preparation and acquisition times. Here we show that X-ray phase-contrast tomography using a single grating can provide a powerful alternative to commonly employed techniques, such as high-resolution episcopic microscopy (HREM). We present the phase tomography of a mouse embryo in paraffin obtained with an X-ray single-grating interferometer at I13-2 Beamline at Diamond Light Source and discuss the results in comparison with HREM measurements. The excellent contrast and quantitative density information achieved non-destructively and without staining using a simple, robust setup make X-ray single-grating interferometry an optimum candidate for high-throughput imaging of biological specimens as an alternative for existing methods like HREM.
    Original languageEnglish
    Pages (from-to)1257
    JournalBiomedical Optics Express
    Volume8
    Issue number2
    Early online date31 Jan 2017
    DOIs
    Publication statusPublished - 1 Feb 2017

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