Yeast Genetics to Investigate the Function of Core Pre-mRNA Splicing Factors

Raymond T. O'Keefe, Jean D. Beggs

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    The yeast Saccharomyces cerevisiae has been used extensively to investigate the functions of RNA and protein components of the spliceosome. As many of the yeast core splicing factors are evolutionarily conserved with human counterparts, information gained in yeast can be applied directly to humans. The ease with which budding yeast can be manipulated genetically has resulted in numerous resources that make genetic analysis in this organism routine. The function of any gene that codes for a spliceosomal component can be analyzed in yeast, and its genetic interactions investigated using genetic screens. Synthetic enhancement or suppression occur when a mutation in one gene respectively exacerbates or alleviates the severity of a mutation in another gene, indicating a functional interaction. In the case of splicing factors, this can lead to new information on the mechanisms of splicing. In this chapter, methods are described for the functional analysis and synthetic enhancement/lethality screens in yeast. © 2012 Wiley-VCH Verlag GmbH & Co. KGaA.
    Original languageEnglish
    Title of host publicationAlternative pre-mRNA Splicing: Theory and Protocols|Altern. pre-mRNA Splicing: Theory and Protocols
    Place of PublicationGermany
    PublisherJohn Wiley & Sons Ltd
    Pages428-436
    Number of pages8
    Edition1
    DOIs
    Publication statusPublished - 2 Feb 2012

    Keywords

    • Plasmid shuffle
    • Pre-mRNA splicing
    • Saccharomyces cerevisiae
    • Spliceosome
    • Synthetic enhancement
    • Synthetic lethality
    • Yeast genetics

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