Yeast glyoxalase I. Circular dichroic spectra and pH effects

Kenneth T. Douglas; Andrew P. Seddon; Yashushi Nakagawa

doi:10.1016/0020-711X(86)90167-9

Yeast glyoxalase I. Circular dichroic spectra and pH effects

Kenneth T. Douglas^*
, Andrew P. Seddon
, Yashushi Nakagawa

^*Corresponding author for this work

Pharmacy

The University of Essex
Michael Reese Hospital

Research output: Contribution to journal › Article › peer-review

Abstract

1. 1. Large scale isolation and physicochemical characterisation of yeast glyoxalase I showed that this enzyme contained small amounts of carbohydrates. 2. 2. Circular dichroic spectra of the enzyme measured in the presence and absence of S-(p-bromobenzyl)glutathione indicated perturbation of a tyrosine on binding of this competitive inhibitor. 3. 3. Values of K_i for competitive inhibitors were pH invariant over the accessible pH range.

Original language	English
Pages (from-to)	549-555
Number of pages	7
Journal	International journal of biochemistry
Volume	18
Issue number	6
DOIs	https://doi.org/10.1016/0020-711X(86)90167-9
Publication status	Published - 1986

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title = "Yeast glyoxalase I. Circular dichroic spectra and pH effects",

abstract = "1. 1. Large scale isolation and physicochemical characterisation of yeast glyoxalase I showed that this enzyme contained small amounts of carbohydrates. 2. 2. Circular dichroic spectra of the enzyme measured in the presence and absence of S-(p-bromobenzyl)glutathione indicated perturbation of a tyrosine on binding of this competitive inhibitor. 3. 3. Values of Ki for competitive inhibitors were pH invariant over the accessible pH range.",

author = "Douglas, \{Kenneth T.\} and Seddon, \{Andrew P.\} and Yashushi Nakagawa",

year = "1986",

doi = "10.1016/0020-711X(86)90167-9",

language = "English",

volume = "18",

pages = "549--555",

journal = "International journal of biochemistry",

issn = "0020-711X",

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number = "6",

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T1 - Yeast glyoxalase I. Circular dichroic spectra and pH effects

AU - Douglas, Kenneth T.

AU - Seddon, Andrew P.

AU - Nakagawa, Yashushi

PY - 1986

Y1 - 1986

N2 - 1. 1. Large scale isolation and physicochemical characterisation of yeast glyoxalase I showed that this enzyme contained small amounts of carbohydrates. 2. 2. Circular dichroic spectra of the enzyme measured in the presence and absence of S-(p-bromobenzyl)glutathione indicated perturbation of a tyrosine on binding of this competitive inhibitor. 3. 3. Values of Ki for competitive inhibitors were pH invariant over the accessible pH range.

AB - 1. 1. Large scale isolation and physicochemical characterisation of yeast glyoxalase I showed that this enzyme contained small amounts of carbohydrates. 2. 2. Circular dichroic spectra of the enzyme measured in the presence and absence of S-(p-bromobenzyl)glutathione indicated perturbation of a tyrosine on binding of this competitive inhibitor. 3. 3. Values of Ki for competitive inhibitors were pH invariant over the accessible pH range.

UR - https://www.scopus.com/pages/publications/0022574523

U2 - 10.1016/0020-711X(86)90167-9

DO - 10.1016/0020-711X(86)90167-9

M3 - Article

C2 - 3519311

AN - SCOPUS:0022574523

SN - 0020-711X

VL - 18

SP - 549

EP - 555

JO - International journal of biochemistry

JF - International journal of biochemistry

IS - 6

ER -

Yeast glyoxalase I. Circular dichroic spectra and pH effects

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