@article{3a7e7dd298a44139b7f49d2bab3e34ce,
title = "Yeast Sen1 helicase protects the genome from transcription-associated instability",
abstract = "Sen1 of S. cerevisiae is a known component of the NRD complex implicated in transcription termination of nonpolyadenylated as well as some polyadenylated RNA polymerase II transcripts. We now show that Sen1 helicase possesses a wider function by restricting the occurrence of RNA:DNA hybrids that may naturally form during transcription, when nascent RNA hybridizes to DNA prior to its packaging into RNA protein complexes. These hybrids displace the nontranscribed strand and create R loop structures. Loss of Sen1 results in transient R loop accumulation and so elicits transcription-associated recombination. SEN1 genetically interacts with DNA repair genes, suggesting that R loop resolution requires proteins involved in homologous recombination. Based on these findings, we propose that R loop formation is a frequent event during transcription and a key function of Sen1 is to prevent their accumulation and associated genome instability.",
author = "Mischo, {Hannah E.} and Bel{\'e}n G{\'o}mez-Gonz{\'a}lez and Pawel Grzechnik and Rond{\'o}n, {Ana G.} and Wu Wei and Lars Steinmetz and Andr{\'e}s Aguilera and Proudfoot, {Nick J.}",
note = "Funding Information: Thanks to our lab colleagues, especially Monika Gullerova and Jurgi Camblong, Julien Gagneur, Sandra Clauder-Muenster, and Charles Giradot (submission to ArrayExpress). Funding was from the Wellcome Trust to N.J.P., the Spanish Ministry of Science and Innovation (BFU2006-05260 and Consolider Ingenio 2010 CSD2007-0015) and Junta de Andaluc{\'i}a (BIO102 and CV2549) to A.A., and a joint ESF grant to N.J.P. and A.A. (NuRNASu). H.E.M. was recipient of a B.I.F. studentship and a European Molecular Biology Organization (EMBO) short-term fellowship. B.G. received a predoctoral FPU training grant from the Spanish Ministry of Science and Innovation. P.G. was supported by a long-term EMBO fellowship. ",
year = "2011",
month = jan,
day = "7",
doi = "10.1016/j.molcel.2010.12.007",
language = "English",
volume = "41",
pages = "21--32",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "1",
}