Projects per year
Abstract
The suite of biological catalysts found in Nature has the potential to contribute immensely to scientific advancements, ranging from industrial biotechnology to innovations in bioenergy and medical intervention. The endeavour to obtain a catalyst of choice is, however, wrought with challenges. Herein we report the design of a structure-based annotation system for the identification of functionally similar enzymes from diverse sequence backgrounds. Focusing on an enzymatic activity with demonstrated synthetic and therapeutic relevance, five new phenylalanine ammonia lyase (PAL) enzymes were discovered and characterised with respect to their potential applications. The variation and novelty of various desirable traits seen in these previously uncharacterised enzymes demonstrates the importance of effective sequence annotation in unlocking the potential diversity that Nature provides in the search for tailored biological tools. This new method has commercial relevance as a strategy for assaying the 'evolvability' of certain enzyme features, thus streamlining and informing protein engineering efforts.
Original language | English |
---|---|
Article number | 13691 |
Journal | Scientific Reports |
Volume | 7 |
Issue number | 1 |
Early online date | 20 Oct 2017 |
DOIs | |
Publication status | Published - 2017 |
Research Beacons, Institutes and Platforms
- Manchester Institute of Biotechnology
Fingerprint
Dive into the research topics of 'Zymophore identification enables the discovery of novel phenylalanine ammonia lyase enzymes'. Together they form a unique fingerprint.Projects
- 1 Finished
-
Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals
Scrutton, N. (PI), Azapagic, A. (CoI), Balmer, A. (CoI), Barran, P. (CoI), Breitling, R. (CoI), Delneri, D. (CoI), Dixon, N. (CoI), Faulon, J.-L. (CoI), Flitsch, S. (CoI), Goble, C. (CoI), Goodacre, R. (CoI), Hay, S. (CoI), Kell, D. (CoI), Leys, D. (CoI), Lloyd, J. (CoI), Lockyer, N. (CoI), Martin, P. (CoI), Micklefield, J. (CoI), Munro, A. (CoI), Pedrosa Mendes, P. (CoI), Randles, S. (CoI), Salehi Yazdi, F. (CoI), Shapira, P. (CoI), Takano, E. (CoI), Turner, N. (CoI) & Winterburn, J. (CoI)
14/11/14 → 13/05/20
Project: Research
Activities
- 1 Organising a conference, workshop, exhibition, performance, inquiry, course etc
-
Manchester Science Festival 2018: Music & Mutation
Weise, N. (Co-Organiser)
27 Oct 2018Activity: Participating in or organising event(s) › Organising a conference, workshop, exhibition, performance, inquiry, course etc › Research
File